Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
Arch Microbiol. 2022 May 9;204(6):306. doi: 10.1007/s00203-022-02915-9.
Crude oil degradation efficiency can be improved because of co-metabolism that exists when bacterial consortium is applied. However, because of possible vulnerability to environmental conditions and/or antagonistic interactions among members of the consortium, the degradation efficiency can be hampered. In this laboratory-based study, the biodegradation potentials of pure bacterial isolates namely Pseudomonas aeruginosa strain W15 (MW320658), Providencia vermicola strain W8 (MW320661) and Serratia marcescens strain W13 (MW320662) earlier isolated from crude oil-contaminated site and their consortium were evaluated using 3% crude oil-supplemented Bushnell Haas media. The efficiency was evaluated based on the viable cell count, biosurfactant analyses, percentage hydrocarbon degradation using gravimetric analysis and gas chromatography-mass spectrophotometry (GC-MS) analysis. There was decline in the population of W13 and predominance of W15 in the consortium as the incubation period progressed. Accelerated biodegradation of the crude oil hydrocarbons through co-metabolism was not achieved with the consortium; neither was there any improved resilience nor resistance to environmental changes of strain W13. The GC-MS analyses showed that the highest degradation was produced by W15 (48.23%) compared to W8 (46.04%), W13 (45.24%) and the Consortium (28.51%). The biodegradation of the crude oil hydrocarbons by W15, W8, W13 axenic cultures and their consortium treatments demonstrated that the bacterial constituent in a consortium can influence the synergistic effect that improves bioremediation. Future research that focuses on evaluating possible improvement in bioremediation through maintenance of diversity by continuous bioaugmentation using vulnerable but efficient degraders in a consortium is necessary to further understand the application of consortia for bioremediation improvement.
原油降解效率可以通过共代谢得到提高，当应用细菌联合体时就会存在共代谢。然而，由于联合体成员可能容易受到环境条件和/或拮抗相互作用的影响，降解效率可能会受到阻碍。在这项基于实验室的研究中，评估了先前从受原油污染的地点分离的纯细菌分离株即铜绿假单胞菌菌株 W15（MW320658）、韦荣氏球菌菌株 W8（MW320661）和粘质沙雷氏菌菌株 W13（MW320662）及其联合体的生物降解潜力，使用 3%的原油补充 Bushnell Haas 培养基。效率是基于活菌计数、生物表面活性剂分析、使用重量分析和气相色谱-质谱法（GC-MS）分析的烃降解百分比来评估的。随着孵育期的进行，联合体中 W13 的数量减少，而 W15 占优势。联合体未能实现通过共代谢加速原油烃的生物降解；也没有改善 W13 对环境变化的弹性或抗性。GC-MS 分析表明，与 W8（46.04%）、W13（45.24%）和联合体（28.51%）相比，W15 产生的降解最高（48.23%）。W15、W8、W13 无菌培养物及其联合体处理对原油烃的生物降解表明，联合体中的细菌成分可以影响协同作用，从而改善生物修复。未来的研究需要集中在通过使用联合体中脆弱但有效的降解剂进行连续生物增强来维持多样性，从而评估通过生物增强来进一步改善生物修复的可能性，这对于进一步理解联合体在生物修复中的应用是必要的。
