Feng Leiyu, Jiang Xiupeng, Huang Yanning, Wen Dongdong, Fu Tianyu, Fu Rongbing
State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai, 200092, China.
State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai, 200092, China; School of Environmental and Safety Engineering, Changzhou University, 1 Gehu Road, Changzhou, Jiangsu Province, 214500, China.
Environ Pollut. 2021 Jan 11;273:116476. doi: 10.1016/j.envpol.2021.116476.
Pollution in soil by petroleum hydrocarbon has become a global environmental problem. The bioremediation of petroleum hydrocarbon-contaminated soil was enhanced with the combination of an isolated indigenous bacterial consortium and biosurfactant. The biodegradation efficiency of total petroleum hydrocarbon (TPH) was increased from 12.2% in the contaminated soil to 44.5% and 57.7% in isolated consortium and isolated consortium & 1.5 g sophorolipid (SL)/kg dry soil, respectively. The half-life of TPH degradation process was decreased from 32.5 d in the isolated consortium reactor to 20.4 d in the isolated consortium & 1.5 g SL/kg dry soil. The addition of biosurfactant into contaminated soils improved the TPH desorption from solid matrix to the aqueous solution and the subsequent solubilization, which ultimately improved the bioavailability of TPH in contaminated soils. Biosurfactant also served as carbon sources which contributed to the stimulation of cell growth and microbial activity and accelerated the biodegradation process via co-metabolism. The enzyme activities and quantities of functional genes were demonstrated to be incremented in SL reactors. The biosurfactant improved the TPH bioavailability, stimulated the microbial activities and participated in the co-metabolism. The combination of bioaugmentation and SL benefitted the bioremediation of petroleum hydrocarbon-contaminated soil.
石油烃对土壤的污染已成为一个全球性环境问题。分离得到的本地细菌群落与生物表面活性剂相结合，提高了石油烃污染土壤的生物修复效果。总石油烃（TPH）的生物降解效率从污染土壤中的12.2%分别提高到分离群落中的44.5%以及分离群落与1.5 g槐糖脂（SL）/kg干土组合体系中的57.7%。TPH降解过程的半衰期从分离群落反应器中的32.5天缩短至分离群落与1.5 g SL/kg干土组合体系中的20.4天。向污染土壤中添加生物表面活性剂可改善TPH从固体基质到水溶液的解吸及随后的增溶作用，最终提高污染土壤中TPH的生物可利用性。生物表面活性剂还作为碳源，有助于刺激细胞生长和微生物活性，并通过共代谢加速生物降解过程。结果表明，SL反应器中的酶活性和功能基因数量均有所增加。生物表面活性剂提高了TPH的生物可利用性，刺激了微生物活性并参与了共代谢。生物强化与SL相结合有利于石油烃污染土壤的生物修复。
