College of Public Health, Capital Medical University. No.10 West, You'anmen Avenue, Fengtai District, Beijing 100069, PR China; Institute for Immunization and Prevention, Beijing Center for Disease Control and Prevention, Beijing Research Center for Preventive Medicine, No.16 Hepingli Middle Street, Dongcheng District, Beijing 100013, PR China.
Institute for Immunization and Prevention, Beijing Center for Disease Control and Prevention, Beijing Research Center for Preventive Medicine, No.16 Hepingli Middle Street, Dongcheng District, Beijing 100013, PR China.
J Infect. 2022 Jul;85(1):75-85. doi: 10.1016/j.jinf.2022.04.046. Epub 2022 May 6.
To investigate the genetic variability and the epidemiological features of respiratory syncytial virus (RSV) in Beijing during five consecutive seasons from 2015 to 2019.
We collected 36,927 samples (ages ranged from 1 day to 101 years old) from cases with acute respiratory tract infections (ARTI) using the Respiratory Pathogens Surveillance System (RPSS) in Beijing, 2015-2019. G gene sequencing and phylogenetic analysis were performed to identify RSV genotypes, clusters, and amino acid (aa) changes.
In total, 764 (2.1%, 764/36927) cases were RSV positive, 52.1% cases were children under 5 years old, and 25.8% were elderly ≥ 60 years old. We obtained 369 sequences of the G gene. ON1 and BA9 were the dominant genotypes in Beijing. Sub-lineage 4 of ON1, which contains four aa substitutions (T113I, N178G, H258Q, and H266L), emerged in 2017 and became the predominant variant in 2018-2019. Sub-lineage 4 of BA9, which contains two aa changes (A131T, T137I), emerged in 2017 and became the predominant variant in 2019. We also observed 10 rarely reported nucleotide deletions in the 3' end of the G gene from five sequences of the ON1 genotype.
With the exception of children < 5 years old, RSV infection mainly occurred in the elderly ≥ 60 years old. Newly emerged sub-lineages have replaced existing sub-lineage over time and become predominant in Beijing. Continued surveillance of the genetic diversity of RSV is necessary.
研究 2015 年至 2019 年连续五个季节北京地区呼吸道合胞病毒（RSV）的遗传变异和流行病学特征。
我们使用北京呼吸道病原体监测系统（RPSS）收集了 36927 例急性呼吸道感染（ARTI）病例的样本（年龄范围为 1 天至 101 岁）。进行 G 基因测序和系统进化分析以确定 RSV 基因型、聚类和氨基酸（aa）变化。
共发现 764 例（2.1%，764/36927）RSV 阳性病例，52.1%的病例为 5 岁以下儿童，25.8%为≥60 岁的老年人。我们获得了 369 条 G 基因序列。ON1 和 BA9 是北京的主要基因型。ON1 的亚谱系 4 包含四个 aa 突变（T113I、N178G、H258Q 和 H266L），于 2017 年出现，并在 2018-2019 年成为主要变异体。BA9 的亚谱系 4 包含两个 aa 变化（A131T、T137I），于 2017 年出现，并在 2019 年成为主要变异体。我们还观察到 5 条 ON1 基因型序列的 G 基因 3'端存在 10 个罕见报道的核苷酸缺失。
除 5 岁以下儿童外，RSV 感染主要发生在≥60 岁的老年人中。新出现的亚谱系随着时间的推移取代了现有的亚谱系，并在北京成为主要变异体。有必要对 RSV 的遗传多样性进行持续监测。
