Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Research Unit of Critical Infection in Children, Chinese Academy of Medical Sciences, 2019RU016, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China.
The 2nd Affiliated Hospital, Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China.
Arch Virol. 2024 Oct 10;169(11):219. doi: 10.1007/s00705-024-06138-9.
Acute lower respiratory tract infections (ALRTIs) are a leading cause of mortality in young children worldwide due to human respiratory syncytial virus (RSV). The aim of this study was to monitor genetic variations in RSV and provide genomic data support for RSV prevention and control. A total of 105 complete RSV genome sequences were determined during 2017-2020. Phylogenetic analysis showed that all of the RSVA sequences were of genotype ON1, and all of the RSVB sequences were of genotype BA9. Notably, a phylogenetic tree based on the whole genome had more branches than a tree based on the G gene. In comparison to the RSV prototype sequences, 71.43% (50/70) of the ON1 sequences had five amino acid substitutions (T113I, V131N, N178G, H258Q, and H266L) that occurred simultaneously, and 68.57% (24/35) of the BA9 genotype sequences had 12 amino acid substitutions, four of which (A131T, T137I, T288I, and T310I) occurred simultaneously. In the F gene, there were 19 amino acid substitutions, which were mainly located in the antigenic sites Ø, II, V, and VII. Other amino acid substitutions were found in the NS1, NS2, P, SH, and L proteins. No significant evidence of recombination was found in any of the sequences. These findings provide important data that will be useful for prevention, control, and vaccine development against RSV.
急性下呼吸道感染（ALRTIs）是全球导致幼儿死亡的主要原因，这是由于人类呼吸道合胞病毒（RSV）所致。本研究旨在监测 RSV 的遗传变异，为 RSV 的预防和控制提供基因组数据支持。在 2017-2020 年期间共确定了 105 例完整的 RSV 基因组序列。系统进化分析表明，所有的 RSV-A 序列均为 ON1 基因型，所有的 RSV-B 序列均为 BA9 基因型。值得注意的是，基于全基因组构建的系统进化树比基于 G 基因构建的树具有更多的分支。与 RSV 原型序列相比，ON1 序列中有 71.43%（50/70）的序列同时发生了 5 个氨基酸替换（T113I、V131N、N178G、H258Q 和 H266L），而 BA9 基因型序列中有 68.57%（24/35）的序列发生了 12 个氨基酸替换，其中 4 个（A131T、T137I、T288I 和 T310I）同时发生。在 F 基因中，有 19 个氨基酸替换，主要位于抗原位点 Ø、II、V 和 VII 中。在 NS1、NS2、P、SH 和 L 蛋白中也发现了其他氨基酸替换。在任何序列中均未发现明显的重组证据。这些发现为 RSV 的预防、控制和疫苗开发提供了重要数据。
