葡萄牙 2014-2018 年呼吸道合胞病毒的流行病学和遗传变异。

Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014-2018.

机构信息

Department of Infectious Diseases, National Health Institute Doutor Ricardo Jorge (INSA), Avenida Padre Cruz, 1649-016, Lisbon, Portugal; European Centre for Disease Prevention and Control (ECDC), Gustav III:s Boulevard 40, 16973, Solna, Sweden.

Department of Infectious Diseases, National Health Institute Doutor Ricardo Jorge (INSA), Avenida Padre Cruz, 1649-016, Lisbon, Portugal.

出版信息

J Clin Virol. 2019 Dec;121:104200. doi: 10.1016/j.jcv.2019.104200. Epub 2019 Oct 10.

INTRODUCTION

Respiratory syncytial virus (RSV) is associated with substantial morbidity and mortality since it is a predominant viral agent causing respiratory tract infections in infants, young children and the elderly. Considering the availability of the RSV vaccines in the coming years, molecular understanding in RSV is necessary.

OBJECTIVE

The objective of the present study was to describe RSV epidemiology and genotype variability in Portugal during the 2014/15-2017/18 period.

MATERIAL AND METHODS

Epidemiological data and RSV-positive samples from patients with a respiratory infection were collected through the non-sentinel and sentinel influenza surveillance system (ISS). RSV detection, subtyping in A and B, and sequencing of the second hypervariable region (HVR2) of G gene were performed by molecular methods. Phylogenetic trees were generated using the Neighbor-Joining method and p-distance model on MEGA 7.0.

RESULTS

RSV prevalence varied between the sentinel (2.5%, 97/3891) and the non-sentinel ISS (20.7%, 3138/16779), being higher (P < 0.0001) among children aged <5 years. Bronchiolitis (62.9%, 183/291) and influenza-like illness (24.6%, 14/57) were associated (P < 0.0001) with RSV laboratory confirmation among children aged <6 months and adults ≥65 years, respectively. The HVR2 was sequenced for 562 samples. RSV-A (46.4%, 261/562) and RSV-B (53.6%, 301/562) strains clustered mainly to ON1 (89.2%, 233/261) and BA9 (92%, 277/301) genotypes, respectively, although NA1 and BA10 were also present until 2015/2016.

CONCLUSION

The sequence and phylogenetic analysis reflected the relatively high diversity of Portuguese RSV strains. BA9 and ON1 genotypes, which have been circulating in Portugal since 2010/2011 and 2011/2012 respectively, predominated during the whole study period.

简介

呼吸道合胞病毒（RSV）是一种主要的病毒病原体，会导致婴儿、幼儿和老年人的呼吸道感染，因此与大量发病率和死亡率有关。考虑到未来几年 RSV 疫苗的可用性，对 RSV 的分子认识是必要的。

目的

本研究的目的是描述 2014/15-2017/18 年期间葡萄牙的 RSV 流行病学和基因型变异情况。

材料和方法

通过非哨点和哨点流感监测系统（ISS）收集患有呼吸道感染的患者的流行病学数据和 RSV 阳性样本。通过分子方法进行 RSV 检测、A 和 B 亚型分型以及 G 基因第二高变区（HVR2）测序。使用邻接法和 MEGA 7.0 上的 p-距离模型生成系统发育树。

结果

哨点（2.5%，97/3891）和非哨点 ISS（20.7%，3138/16779）之间的 RSV 流行率存在差异，<5 岁儿童的流行率更高（P<0.0001）。<6 个月儿童中与 RSV 实验室确诊相关的疾病主要为细支气管炎（62.9%，183/291），而≥65 岁成人中与 RSV 实验室确诊相关的疾病主要为流感样疾病（24.6%，14/57）（P<0.0001）。对 562 个样本进行了 HVR2 测序。RSV-A（46.4%，261/562）和 RSV-B（53.6%，301/562）株主要聚类到 ON1（89.2%，233/261）和 BA9（92%，277/301）基因型，尽管直到 2015/2016 年仍存在 NA1 和 BA10 基因型。