Wang Jie, Chen Zhangfan, Li Ming, Song Yu, Xu Wenteng, Wang Lei, Chen Songlin
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China; Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao, 266071, China.
Fish Shellfish Immunol. 2022 May;124:513-524. doi: 10.1016/j.fsi.2022.04.026. Epub 2022 Apr 25.
Interleukin 10 (IL-10), a pleiotropic cytokine, plays an essential role in multiple immunity responses. In the current study, the sequences of IL-10 family were identified from spotted knifejaw (Oplegnathus punctatus) whole genome, and O. punctatus IL-10 (OpIL-10) was cloned and characterized. OpIL-10 encodes 187 amino acids with a typical IL-10 family signature motif and predicted α-helices. It shared high identities with Notolabrus celidotus IL-10 and Epinephelus Lanceolatus IL-10. OpIL-10 was widely detected in healthy tissues, with the abundant expression in liver and skin. It was significantly up-regulated in the six immune-related tissues (liver, spleen, kidney, intestine, gill and skin) after infection against Vibrio harveyi and spotted knifejaw iridovirus (SKIV). Dual-luciferase analysis showed that OpIL-10 overexpression could suppress the activity of NF-κB. Meanwhile, OpIL-10 knockdown caused the down-regulation of five immune-related genes in JAK2/STAT3 signaling pathway and NF-κB signaling pathway, including IL-10R2, TYK2, STAT3, NOD2, and IκB. In addition, LPS and poly I:C stimulated expression of pro-inflammatory cytokines, including IL-6, IL-1β, IL-8, and IL-12, were lower with recombinant OpIL-10 (rOp IL-10) than the control group, indicating the anti-inflammatory roles of rOpIL-10. Taken together, these results indicated OpIL-10 as a negative regulator in the inflammatory responses of spotted knifejaw against bacterial and viral infection, which would help us better understand the role of IL-10 in teleost immunity.
白细胞介素10（IL-10）是一种多效性细胞因子，在多种免疫反应中发挥着重要作用。在本研究中，从斑石鲷（Oplegnathus punctatus）全基因组中鉴定出IL-10家族序列，并对斑石鲷IL-10（OpIL-10）进行了克隆和特征分析。OpIL-10编码187个氨基酸，具有典型的IL-10家族特征基序和预测的α螺旋。它与新西兰红鱼（Notolabrus celidotus）IL-10和鞍带石斑鱼（Epinephelus Lanceolatus）IL-10具有高度同源性。OpIL-10在健康组织中广泛表达，在肝脏和皮肤中表达丰富。在感染哈维氏弧菌（Vibrio harveyi）和斑石鲷虹彩病毒（SKIV）后，其在六个免疫相关组织（肝脏、脾脏、肾脏、肠道、鳃和皮肤）中显著上调。双荧光素酶分析表明，OpIL-10过表达可抑制NF-κB的活性。同时，敲低OpIL-10导致JAK2/STAT3信号通路和NF-κB信号通路中五个免疫相关基因的下调，包括IL-10R2、TYK2、STAT3、NOD2和IκB。此外，重组OpIL-10（rOpIL-10）处理组中，脂多糖（LPS）和聚肌胞苷酸（poly I:C）刺激的促炎细胞因子（包括IL-6、IL-1β、IL-8和IL-12）的表达低于对照组，表明rOpIL-10具有抗炎作用。综上所述，这些结果表明OpIL-10是斑石鲷对抗细菌和病毒感染的炎症反应中的负调节因子，这将有助于我们更好地理解IL-10在硬骨鱼免疫中的作用。
