Song Yu, Wang Lei, Li Kaimin, Zhang Mengqian, Chen Songlin
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao 266237, China.
Animals (Basel). 2025 Mar 31;15(7):1006. doi: 10.3390/ani15071006.
This study investigates the role of the , , and genes in antiviral and antibacterial immunity of spotted knifejaw (). The expression patterns of these genes were analyzed using qRT-PCR in different tissues and at different time points. The open reading frame (ORF) of the gene was 2757 bp in length and encoded 918 amino acids, the ORF of the gene was 2970 bp in length and encoded 990 amino acids, while the gene was 2172 bp in length and encoded 723 amino acids. The Opnod1 and Opnod2 proteins contained three conserved domains (CARD, NOD, and LRR), and Optbk1 contained an STKc domain. The , and genes were mainly expressed in immune-related tissues of spotted knifejaw, with the highest relative expression of the in the skin, the in the gill, and the in the liver. The expression of these genes changed significantly in the immune tissues following infection with SKIV-SD and . In kidney cells, the , , and expression was up-regulated after stimulation by poly I:C and LPS in vitro. The results suggest that the NOD1/2-TBK1 signal pathway may play an important role in the resistance of the spotted knifejaw to virus and bacteria, providing valuable insights for disease-resistant breeding.
本研究调查了 、 和 基因在斑石鲷抗病毒和抗菌免疫中的作用。使用qRT-PCR分析了这些基因在不同组织和不同时间点的表达模式。 基因的开放阅读框（ORF）长度为2757 bp，编码918个氨基酸， 基因的ORF长度为2970 bp，编码990个氨基酸，而 基因长度为2172 bp，编码723个氨基酸。Opnod1和Opnod2蛋白包含三个保守结构域（CARD、NOD和LRR），Optbk1包含一个STKc结构域。 、 和 基因主要在斑石鲷的免疫相关组织中表达，其中 在皮肤中的相对表达最高， 在鳃中最高， 在肝脏中最高。在用SKIV-SD和 感染后，这些基因在免疫组织中的表达发生了显著变化。在肾细胞中，体外经聚肌胞苷酸（poly I:C）和脂多糖（LPS）刺激后， 、 和 的表达上调。结果表明，NOD1/2-TBK1信号通路可能在斑石鲷对病毒和细菌的抗性中发挥重要作用，为抗病育种提供了有价值的见解。
