Saha Amit, Asaduzzaman Mohammad, Bari Farida Yeasmin
Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Department of Livestock Services, Farmgate, Dhaka 1215, Dhaka, Bangladesh.
Vet Med Int. 2022 Apr 30;2022:7378379. doi: 10.1155/2022/7378379. eCollection 2022.
Germplasm storage and transportation in artificial insemination (AI) and other advanced technologies are facilitated by cryopreservation. In reproduction, the cryopreservation of sperm allows it to be transported across vast distances and used even after the sire's death. However, the technique of cryopreservation might damage sperm and limit their activity. Several cryobiological investigations have reported that the integrity of the sperm membrane is frequently involved in the physical and biological elements that affect sperm survival at low temperatures during the cryopreservation process. However, successful cryopreservation of ram sperm is still a work in progress because a considerable percentage of sperm do not survive the freezing and thawing process. Sperms are destroyed during cryopreservation of semen due to varying concentrations of cryoprotective chemicals and if semen is not cooled at optimal cooling rates. Hence, it is crucial to know the optimum cooling rates with freezing and thawing protocols for maximum recovery of viable and functional sperm cells for a successful cryo-freezing of ram spermatozoa. Therefore, the current study compiled and compared the research on the impact of different cryopreservation procedures, cooling rates, equilibration time, and thawing protocols on post-thaw ram semen quality.
冷冻保存有助于人工授精（AI）及其他先进技术中的种质储存和运输。在繁殖过程中，精子的冷冻保存使其能够远距离运输，甚至在种公羊死亡后仍可使用。然而，冷冻保存技术可能会损害精子并限制其活性。多项低温生物学研究报告称，精子膜的完整性经常涉及影响冷冻保存过程中精子在低温下存活的物理和生物因素。然而，公羊精子的成功冷冻保存仍在进行中，因为相当比例的精子在冷冻和解冻过程中无法存活。由于冷冻保护化学物质浓度不同，以及精液未以最佳冷却速率冷却，精液冷冻保存过程中精子会被破坏。因此，了解最佳冷却速率以及冷冻和解冻方案对于最大程度地恢复有活力和功能的精子细胞以成功冷冻公羊精子至关重要。因此，本研究汇总并比较了不同冷冻保存程序、冷却速率、平衡时间和解冻方案对解冻后公羊精液质量影响的研究。
