Dayanıklı Cemal, Bülbül Bülent, Doğan Şükrü, Şengül Ebru, Kırbaş Mesut, Kal Yavuz, Ataman Mehmet Bozkurt
Department of Breeding Techniques, Sheep Breeding Research Institute, Balıkesir, Türkiye.
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Dokuz Eylül University, İzmir, Türkiye.
Vet Med Sci. 2025 May;11(3):e70381. doi: 10.1002/vms3.70381.
Ram semen cryotolerance problem continues despite intensive research. This study aimed to evaluate the effect of replacing total seminal plasma (SP) with that of high-cryotolerant rams or extender on semen quality parameters in low-cryotolerant rams. Rams previously determined as low (n = 6) and high-cryotolerant (n = 5) were used in the breeding season. High-cryotolerant rams were only included with their SP in the SP+ group. Each normospermic fresh ejaculate from low-cryotolerant rams was split into three aliquots to form the groups. The control aliquot was diluted using a two-step process. Centrifugation removed SP from the second aliquot (SP- group), and the sperm pellet was rediluted with a Tris-based extender using the same amount of SP removed. The third aliquot's SP was removed as in the SP- group, but the remaining sperm pellet was rediluted with fresh SP harvested from high-cryotolerant rams using the same amounts of removed SP in the SP+ group. Semen was rediluted with a Tris-based extender in the SP- and SP+ groups using a two-step process as in the control group. Sperm quality was similar between the groups after dilution and equilibration. However, the post-thaw semen quality was higher in the SP- (one parameter) and SP+ (four parameters) groups than in the control group (p < 0.05). Additionally, these parameters were similar in the SP- and SP+ groups. This study showed that, although an adequate extender had positive effects, replacing the whole SP with that of high-cryotolerant rams may better solve the low cryotolerance problem in ram semen.
尽管进行了深入研究，但公羊精液的冷冻耐受性问题仍然存在。本研究旨在评估用高冷冻耐受性公羊的精浆或稀释液替代低冷冻耐受性公羊的全部精浆对精液质量参数的影响。在繁殖季节使用先前确定为低冷冻耐受性（n = 6）和高冷冻耐受性（n = 5）的公羊。高冷冻耐受性公羊仅在精浆 + 组中与它们的精浆一起使用。将低冷冻耐受性公羊的每份正常精子新鲜射精液分成三等份以形成各组。对照等分试样采用两步法进行稀释。通过离心从第二等分试样中去除精浆（精浆 - 组），然后用等量去除的精浆用基于Tris的稀释液对精子沉淀进行再稀释。第三等分试样的精浆如在精浆 - 组中那样去除，但剩余的精子沉淀用从高冷冻耐受性公羊收集的新鲜精浆以精浆 + 组中相同的去除精浆量进行再稀释。精浆 - 组和精浆 + 组中的精液使用与对照组相同的两步法用基于Tris的稀释液进行再稀释。稀释和平衡后各组之间的精子质量相似。然而，解冻后精浆 - 组（一个参数）和精浆 + 组（四个参数）的精液质量高于对照组（p < 0.05）。此外，这些参数在精浆 - 组和精浆 + 组中相似。本研究表明，尽管适当的稀释液有积极作用，但用高冷冻耐受性公羊的精浆替代整个精浆可能更好地解决公羊精液冷冻耐受性低的问题。
