Sigit Bintara, Maharani Dyah, Tavares Luis, Sitaresmi Pradita Iustitia
Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Faculdade de Agricultura, Universidade Nacional Timor Lorosa-e, Díli 10000, East Timor.
J Anim Sci Technol. 2024 Sep;66(5):891-904. doi: 10.5187/jast.2023.e54. Epub 2024 Sep 30.
The primary cause of sperm quality decline during the freeze-thaw pathway is the peroxidation hazard caused by reactive oxygen species produced by the biological molecules of sperm. Ascorbic acid (Vitamin C) and lycopene are two potent antioxidants that operate to prevent oxidation processes. This study aimed to analyse the effects of ascorbic acid and lycopene on the motility, viability, abnormality and plasma membrane integrity of post-thawed Sapudi rams. Sperm samples were obtained and pooled from six sexually mature Sapudi rams, separated into ten equal proportions and diluted with Tris-egg yolk-glycerol (TEY) extender. Semen was supplemented with 0 (C0; L0), 1 (C1; L1), 2 (C2; L2), 3 (C3; L3) and 4 (C4; L4) mg/100 mL (1%-4%) diluent each of ascorbic acid and lycopene, respectively. Total sperm motility, viability, abnormalities and semen membrane plasma (%) were analysed after thawing. C3 and L3 extenders resulted in higher total motility ( < 0.05) compared to the other extenders, with all treatments higher than that of the control. The extender C3 ( < 0.05) exhibited the highest semen quality. Finally, the current findings show that C3 and L3 can increase the quality of post-thawed Sapudi ram spermatozoa.
冻融过程中精子质量下降的主要原因是精子生物分子产生的活性氧导致的过氧化危害。抗坏血酸（维生素C）和番茄红素是两种有效的抗氧化剂，可防止氧化过程。本研究旨在分析抗坏血酸和番茄红素对解冻后萨普迪公羊精子活力、存活率、畸形率和质膜完整性的影响。从六只性成熟的萨普迪公羊采集精子样本并合并，分成十等份，用Tris-蛋黄-甘油（TEY）稀释液稀释。精液分别添加0（C0；L0）、1（C1；L1）、2（C2；L2）、3（C3；L3）和4（C4；L4）mg/100 mL（1%-4%）的抗坏血酸和番茄红素稀释液。解冻后分析精子总活力、存活率、畸形率和精液质膜百分比（%）。与其他稀释液相比，C3和L3稀释液解冻后的精子总活力更高（<0.05），所有处理组均高于对照组。C3稀释液（<0.05）的精液质量最高。最后，目前的研究结果表明，C3和L3可以提高解冻后萨普迪公羊精子的质量。
