Henan University of Science and Technology, Luoyang, Henan Province 471000, China.
Biomed Res Int. 2022 Apr 30;2022:8592729. doi: 10.1155/2022/8592729. eCollection 2022.
The objective is to investigate the relationship and correlation between PEDF and TGF- in aqueous humor and serum and high myopia CNV lesions.
For each group of patients (namely, group A: patients with high myopia CNV (mCNV); group B: patients with high myopia without CNV; group C: patients with CNV caused by other eye diseases; and group D (control group): patients with simple cataract (without CNV and high myopia)), 20 patients were collected. A total of 40 patients have been collected since the beginning of the study in December 2020, including 7 patients in group A, 13 patients in group B, 10 patients in group C, and 10 patients in group D. Serum and aqueous humor samples were collected, and PEDF and TGF- levels in serum and aqueous humor were detected by enzyme-linked immunosorbent assay (ELISA). SPSS 26.0 statistical software was used to process the data. Independent sample -test was used to compare the data of the same factor in the same group between serum and aqueous humor. Comparisons of the same factors between different groups were performed using a one-way analysis of variance (ANOVA). Correlation analysis was conducted by the Pearson correlation coefficient test. < 0.05 indicated that the difference was statistically significant.
There were no significant differences in age, gender, and course of disease among all groups ( > 0.05). The concentration of PEDF in aqueous humor in group A and group C was higher than that in group B and group D. There was no significant correlation between serum PEDF content and the above-mentioned diseases. The concentration of TGF- in aqueous humor in groups A, B, and C was significantly higher than that in group D. However, there was no significant correlation between TGF- content in serum and the above-mentioned diseases. There was no significant correlation between aqueous humor and serum PEDF. There was no significant correlation between the content of TGF- in aqueous humor and serum.
TGF- in aqueous humor may be involved in the development of high myopia and intraocular CNV disease. However, PEDF in aqueous humor may be involved in the development of intraocular CNV disease and has no significant correlation with high myopia. At the same time, TGF- and PEDF in serum had no significant correlation with high myopia and intraocular CNV disease. There was no significant correlation between the concentrations of TGF- and PEDF in aqueous humor and serum.
探讨 PEDF 与 TGF-β在房水和血清中与高度近视脉络膜新生血管（CNV）病变的关系和相关性。
收集每一组患者（即 A 组：高度近视 CNV 患者；B 组：高度近视无 CNV 患者；C 组：由其他眼病引起的 CNV 患者；D 组（对照组）：单纯白内障患者（无 CNV 和高度近视）），每组 20 例。自 2020 年 12 月研究开始以来共收集了 40 例患者，其中 A 组 7 例，B 组 13 例，C 组 10 例，D 组 10 例。采集血清和房水样本，采用酶联免疫吸附试验（ELISA）检测血清和房水中 PEDF 和 TGF-β水平。采用 SPSS 26.0 统计软件处理数据。采用独立样本 t 检验比较同一组血清与房水同因子的数据。采用单因素方差分析（ANOVA）比较不同组间相同因子的差异。采用 Pearson 相关系数检验进行相关性分析。<0.05 表示差异有统计学意义。
各组间年龄、性别、病程比较差异均无统计学意义（>0.05）。A 组和 C 组房水中 PEDF 浓度高于 B 组和 D 组，血清 PEDF 含量与上述疾病无明显相关性。A、B、C 组房水中 TGF-β浓度明显高于 D 组，但血清 TGF-β含量与上述疾病无明显相关性。房水与血清 PEDF 无明显相关性。房水中 TGF-β含量与血清无明显相关性。
房水中 TGF-β可能参与高度近视和眼内 CNV 疾病的发生。然而，房水中的 PEDF 可能参与眼内 CNV 疾病的发生，与高度近视无明显相关性。同时，血清中 TGF-β和 PEDF 与高度近视和眼内 CNV 疾病无明显相关性。房水和血清中 TGF-β和 PEDF 的浓度无明显相关性。
