McPhail G D, Finn T, Isaacson P G
J Pathol. 1987 Mar;151(3):231-8. doi: 10.1002/path.1711510311.
Using a low temperature resin, we have developed a reliable technique for post-embedding electron immunocytochemistry which is rapid enough to be used in a routine histopathology laboratory. Glutaraldehyde fixed human tissues were dehydrated and embedded at -25 degrees C in a new acrylic resin called LR-Gold. Using the immunogold technique, ultra-thin sections of the tissues were labelled with monoclonal antibodies to cytokeratin, human milk fat globule and HLA-D region antigen; and with polyclonal antisera to lysozyme, kappa and lambda light chains and immunoglobulin M. The resin was easy to section and the preservation of fine structure was excellent. Immunolabelling procedures gave clean and consistent results, and electron micrographs of examples of this are included. It was felt that the preservation of ultrastructure and antigenicity compared well with the results of other workers using low temperature resins such as Lowicryl K4M, but LR-Gold was superior to Lowicryl K4M because sectioning was considerably easier and the sections were more stable in the electron beam.
我们使用一种低温树脂，开发出了一种用于包埋后电子免疫细胞化学的可靠技术，该技术速度足够快，可用于常规组织病理学实验室。用戊二醛固定的人体组织经脱水后，于-25℃包埋在一种名为LR-金的新型丙烯酸树脂中。采用免疫金技术，用针对细胞角蛋白、人乳脂肪球和HLA-D区抗原的单克隆抗体，以及针对溶菌酶、κ和λ轻链及免疫球蛋白M的多克隆抗血清对组织超薄切片进行标记。这种树脂易于切片，细微结构保存良好。免疫标记程序得到了清晰且一致的结果，本文还包含了相关的电子显微镜照片实例。据认为，与其他使用如Lowicryl K4M等低温树脂的研究人员的结果相比，该方法在超微结构和抗原性的保存方面表现良好，但LR-金优于Lowicryl K4M，因为切片要容易得多，且切片在电子束下更稳定。
