Taylor M J, Abdel-Wahab N, Wu Y, Jenkins R E, Bianco A E
Liverpool School of Tropical Medicine, United Kingdom.
Infect Immun. 1995 Nov;63(11):4417-22. doi: 10.1128/iai.63.11.4417-4422.1995.
OvB20 is an antigen of Onchocerca volvulus preferentially recognized by sera from cattle vaccinated with irradiated infective larvae of Onchocerca lienalis. Antibodies raised against the recombinant protein were used to characterize the expression of the native protein in different developmental stages of O. volvulus and the rodent filaria Acanthocheilonema viteae. In O. volvulus, antibodies reacted to a polypeptide of 42 kDa in microfilariae and with proteins of 52 and 65 kDa in third-stage larvae. No products were detected in adult stages. Immunogold electron microscopy localized the native protein to discrete patches of the hypodermis and cuticle of infective larvae. Characterization of a homologous protein in A. viteae confirmed the stage-specific expression in infective larvae of the 65-kDa protein, which was secreted during in vitro culture. Vaccination of rodents against A. viteae with a B20-maltose-binding-protein fusion protein resulted in a 49 to 60% reduction in adult worm recoveries with a corresponding 97% reduction in microfilaremia.
OvB20是旋盘尾丝虫的一种抗原，优先被用辐射致弱的链尾丝虫感染性幼虫免疫的牛血清所识别。针对该重组蛋白产生的抗体被用于表征旋盘尾丝虫不同发育阶段以及啮齿类丝虫——旋盘尾丝虫体内天然蛋白的表达情况。在旋盘尾丝虫中，抗体与微丝蚴中42 kDa的一种多肽发生反应，并与第三期幼虫中52 kDa和65 kDa的蛋白质发生反应。在成虫阶段未检测到产物。免疫金电子显微镜将天然蛋白定位在感染性幼虫的皮下组织和角质层的离散斑块中。对旋盘尾丝虫体内同源蛋白的表征证实了65 kDa蛋白在感染性幼虫中的阶段特异性表达，该蛋白在体外培养期间被分泌。用B20 - 麦芽糖结合蛋白融合蛋白对啮齿动物进行旋盘尾丝虫疫苗接种，导致成虫回收率降低49%至60%，同时微丝蚴血症相应降低97%。
