Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran.
Dis Markers. 2022 Apr 30;2022:1495082. doi: 10.1155/2022/1495082. eCollection 2022.
It has been established that microRNAs (miRNAs) are involved in the regulation of immune responses and serve as biomarkers of inflammatory diseases as well as recurrent spontaneous miscarriage (RSM). Herein, we aimed to study the relationship between three functional gene polymorphisms with idiopathic RSM (IRSM) susceptibility. We recruited 161 patients with IRSM and 177 healthy women with at least one live birth and without a history of abortion. Genotyping was performed using RFLP-PCR and ARMS-PCR methods. We found that the rs6864584 T/C decreased the risk of IRSM under dominant TT+TC vs. CC (OR = 0.029) and allelic C vs. T (OR = 0.028) contrast models. Regarding rs2961920 A/C and rs57095329 A/G polymorphisms, the enhanced risk of IRSM was observed under different genetic contrasted models, including the codominant CC vs. AA (OR = 2.81 for rs2961920) and codominant GG vs. AA (OR = 2.36 for rs57095329). After applying a Bonferroni correction, haplotype analysis revealed a 51% decreased risk of IRSM regarding the ACA genotype combination. This is the first study reporting that rs57095329 A/G, rs2961920A/C, and rs6864584 T/C polymorphisms are associated with the risk of IRSM in a southern Iranian population. Performing replicated case-control studies on other ethnicities is warranted to outline the precise effects of the studied variants on the risk of gestational trophoblastic disorders.
已经证实，微小 RNA（miRNA）参与免疫反应的调节，并且可以作为炎症性疾病以及复发性自然流产（RSM）的生物标志物。在此，我们旨在研究三个功能基因多态性与特发性 RSM（IRSM）易感性之间的关系。我们招募了 161 名 IRSM 患者和 177 名至少有一次活产且无流产史的健康女性。采用 RFLP-PCR 和 ARMS-PCR 方法进行基因分型。我们发现，rs6864584T/C 在显性 TT+TC 与 CC 相比（OR=0.029）和等位基因 C 与 T 相比（OR=0.028）时降低了 IRSM 的风险。关于 rs2961920A/C 和 rs57095329A/G 多态性，在不同的遗传对比模型下观察到 IRSM 的风险增加，包括共显性 CC 与 AA（OR=rs2961920 为 2.81）和共显性 GG 与 AA（OR=rs57095329 为 2.36）。在应用 Bonferroni 校正后，单体型分析显示 ACA 基因型组合的 IRSM 风险降低了 51%。这是第一项报道 rs57095329A/G、rs2961920A/C 和 rs6864584T/C 多态性与伊朗南部人群 IRSM 风险相关的研究。在其他种族中进行复制病例对照研究，以阐明研究变异对妊娠滋养细胞疾病风险的精确影响是有必要的。
