Zhang Hongwei, Qu Yuan, Zhang Yanchao, Yan Yumei, Gao Haixiang
Department of Applied Chemistry, China Agricultural University, Yuanmingyuan West Road 2#, Haidian District, Beijing 100193, China.
Anal Methods. 2022 May 27;14(20):1996-2002. doi: 10.1039/d2ay00237j.
Herein, we report the preparation of a gold nanoparticle-thioglycolic acid (TGA@AuNP) suspension for the label-free colorimetric detection of the organophosphorus pesticide chlorpyrifos. This colorimetric sensor was synthesized by a one-pot method, providing thioglycolic acid (TGA)-modified gold nanoparticles with a negatively charged surface. The formation of TGA@AuNPs was confirmed by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and dynamic light scattering (DLS). In addition, chlorpyrifos was quantitatively determined by UV-vis spectrophotometry. The modification time, TGA concentration, pH, incubation time, temperature, ionic strength, and interference of other pesticides were also investigated. The synthesized TGA@AuNP colorimetric sensor possesses a detection limit as low as 20 μg L and a selective sensing response, and the detection time is less than 2 minutes. In addition, this method can also be applied to detect various practical samples in life, such as environmental water samples and tea.
在此，我们报告了一种用于无标记比色法检测有机磷农药毒死蜱的金纳米粒子-巯基乙酸（TGA@AuNP）悬浮液的制备。这种比色传感器通过一锅法合成，提供了具有带负电荷表面的巯基乙酸（TGA）修饰的金纳米粒子。通过傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）和动态光散射（DLS）确认了TGA@AuNPs的形成。此外，通过紫外可见分光光度法定量测定了毒死蜱。还研究了修饰时间、TGA浓度、pH值、孵育时间、温度、离子强度以及其他农药的干扰。合成的TGA@AuNP比色传感器具有低至20μg/L的检测限和选择性传感响应，检测时间小于2分钟。此外，该方法还可应用于检测生活中的各种实际样品，如环境水样和茶叶。
