Department of Chemistry and Nanoscience, Ewha Womans University, Seoul, 03760, Republic of Korea.
Department of Chemistry of Natural Compounds, University of Chemistry and Technology, Prague, Technická 5, Prague 6, 16628, Czech Republic.
Adv Healthc Mater. 2022 Jul;11(14):e2200529. doi: 10.1002/adhm.202200529. Epub 2022 May 18.
Combating biofilm infections remains a challenge due to the shield and acidic conditions. Herein, an acid-responsive nanoporphyrin (PN3-NP) based on the self-assembly of a water-soluble porphyrin derivative (PN3) is constructed. Additional kinetic control sites formed by the conjugation of the spermine molecules to a porphyrin macrocycle make PN3 self-assemble into stable nanoparticles (PN3-NP) in the physiological environment. Noteworthily, near-infrared (NIR) fluorescence monitoring and synergistic photodynamic therapy (PDT) and photothermal therapy (PTT) effects of PN3-NP can be triggered by the acidity in biofilms, accompanied by intelligent transformation into dot-like nanospheres. Thus, damage to normal tissue is effectively avoided and accurate diagnosis and treatment of biofilms is achieved successfully. The good results of fluorescence imaging-guided photo-ablation of antibiotic-resistant strains methicillin-resistant Staphylococcus aureus (MRSA) biofilms verify that PN3-NP is a promising alternative to antibiotics. Meanwhile, this strategy also opens new horizons to engineer smart nano-photosensitizer for accurate diagnosis and treatment of biofilms.
由于生物膜的屏蔽作用和酸性环境，对抗生物膜感染仍然是一个挑战。本文构建了一种基于水溶性卟啉衍生物（PN3）自组装的酸响应纳米卟啉（PN3-NP）。通过将 spermine 分子与卟啉大环偶联形成额外的动力学控制位点，使 PN3 在生理环境中自组装成稳定的纳米颗粒（PN3-NP）。值得注意的是，生物膜中的酸性可以触发 PN3-NP 的近红外（NIR）荧光监测以及协同光动力疗法（PDT）和光热疗法（PTT）效应，并伴随着智能转化为点状纳米球。因此，有效避免了对正常组织的损伤，实现了生物膜的准确诊断和治疗。荧光成像引导的耐抗生素金黄色葡萄球菌（MRSA）生物膜光消融的良好结果验证了 PN3-NP 是抗生素的一种有前途的替代品。同时，该策略也为工程智能纳米光敏剂用于生物膜的准确诊断和治疗开辟了新的视野。
