Department of Chemistry, Yonsei University, Wonju 26493, Republic of Korea.
Division of Biological Science and Technology, Yonsei University, Wonju 26493, Republic of Korea.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 5;278:121333. doi: 10.1016/j.saa.2022.121333. Epub 2022 May 4.
A probe with an isothiocyanate group was synthesized and evaluated for its HS sensing ability. Upon addition of HS, the probe exhibited ratiometric properties during absorption with a red-shift. The probe exhibited fluorescent off-on responses towards HS via the ESIPT process, due to the conversion of isocyanate into amine. UV-vis, fluorescence, and H NMR spectroscopic analyses were performed to investigate the sensing mechanism. The probe has a large Stokes shift, short response time, and low detection limit. It can be used to estimate HS levels within the range of 0-36 nM. The practical applicability of the probe was demonstrated using water samples and living cells.
合成了一种带有异硫氰酸酯基团的探针，并评估了其对 HS 的传感能力。加入 HS 后，探针在吸收时表现出比率特性，发生红移。探针通过 ESIPT 过程对 HS 表现出荧光开-关响应，这是由于异氰酸酯转化为胺。通过 UV-vis、荧光和 1H NMR 光谱分析研究了传感机制。该探针具有较大的斯托克斯位移、短的响应时间和低的检测限。它可以用于估计 0-36 nM 范围内的 HS 水平。通过水样和活细胞证明了探针的实际应用。
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