A dual-color ESIPT-based probe for simultaneous detection of hydrogen sulfide and hydrazine.

Hydrogen sulfide (HS) and hydrazine (NH) are toxic compounds in environmental and living systems, and hydrogen sulfide is also an important signaling molecule. However, in the absence of dual-color probes capable of detecting both HS and NH, the ability to monitor the crosstalk of these substances is restricted. Herein, we developed an ESIPT-based dual-response fluorescent probe (BDM-DNP) for HS and NH detection dually responsive sites. The BDM-DNP possessed absorbing strength in the detection of HS and NH, with a large Stokes shift (156 nm for HS and 108 nm for NH), high selectivity and sensitivity, and good biocompatibility. Furthermore, BDM-DNP can be utilized for the detection of hydrogen sulfide and hydrazine in actual soil, and gaseous HS and NH in environmental systems. Notably, BDM-DNP can detect HS and NH in living cells for disease diagnosis and treatment evaluation.