College of Civil and Transportation Engineering, Shenzhen University, Shenzhen 518060, China; Archaeal Biology Center, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
Archaeal Biology Center, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
Sci Total Environ. 2022 Sep 10;838(Pt 1):155784. doi: 10.1016/j.scitotenv.2022.155784. Epub 2022 May 9.
In marine environments, increasing occurrence and numbers of microbial Antibiotic Resistance Gene (ARG) subtypes, especially of new beta-lactamases, have received lots of attention in recent years. Updated databases with novel developed tools provide new opportunities to obtain more comprehensive ARG profiles as well as ARG-carrying hosts. Yet, ARGs in human-associated marine aquaculture environments, e.g. in China, remains largely unknown. Using metagenomic data, we revealed high numbers of Multi-drug Resistance, beta-lactamase and aminoglycoside genes throughout the year. Thereby, Alpha- and Gamma-proteobacteria were assigned to the majority of beta-lactamase-carrying hosts. From Metagenome-assembled genomes, three blaF-like beta-lactamases (91.7-94.7% identity with beta-lactamase from Mycobacterium fortuitum (blaF)) were exclusively observed in an unclassified Mycobacterium genus. Notably, other new beta-lactamases, VMB-1-like (n = 3) (58.5-67.4% identity to Vibrio metallo-beta-lactamase 1 (VMB-1)), were found in Gammaproteobacteria. Additionally, 175 Multi-drug Resistant Organisms possessed at least 3 ARG subtypes, and seven of the potentially pathogenic genera (n = 17) were assigned to Gammaproteobacteria. These results, together with high-risk ARGs (e.g. tetM, dfrA14 and dfrA17), provide hosts and new beta-lactamases of ARGs in Chinese coastal aquaculture.
在海洋环境中，微生物抗生素耐药基因（ARG）亚型的出现频率和数量不断增加，尤其是新型β-内酰胺酶，近年来受到了广泛关注。更新的数据库和新开发的工具为获得更全面的 ARG 谱以及携带 ARG 的宿主提供了新的机会。然而，中国沿海水产养殖环境中人类相关的 ARG 仍然知之甚少。本研究利用宏基因组数据，全年都揭示了大量的多药耐药、β-内酰胺酶和氨基糖苷类基因。因此，α-和γ-变形菌被认为是携带β-内酰胺酶的大多数宿主。从宏基因组组装基因组中，我们专门在一个未分类的分枝杆菌属中观察到三种 blaF 样β-内酰胺酶（与分枝杆菌的 blaF 具有 91.7-94.7%的同一性）。值得注意的是，其他新型β-内酰胺酶，如 VMB-1 样（与 Vibrio metallo-beta-lactamase 1（VMB-1）的同一性为 58.5-67.4%），在γ-变形菌中被发现。此外，175 种多药耐药生物至少携带 3 种 ARG 亚型，其中 7 种潜在的致病性属（n = 17）被归入γ-变形菌。这些结果，加上高风险的 ARG（如 tetM、dfrA14 和 dfrA17），为中国沿海水产养殖提供了携带 ARG 的宿主和新型β-内酰胺酶。
