Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China.
The Joint Key Laboratory of Aquatic Products Processing of Zhejiang Province, Hangzhou, China.
Pol J Microbiol. 2023 Mar 24;72(1):61-67. doi: 10.33073/pjm-2023-010. Print 2023 Mar 1.
The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) among the aquatic products collected in Zhejiang, China. A total of 136 presumptive ARB picked up from six aquatic samples were classified into 22 genera and 49 species based on the 16S rDNA sequencing. spp., spp., spp., spp., spp., and spp. accounted for 80% of the ARB. Among them, 109 isolates (80.15%) exhibited resistance to at least one antibiotic. Most isolates showed resistance to not only the originally selected drug but also to one to three other tested drugs. The diversity of ARB distributed in different aquatic products was significant. Furthermore, the resistance data obtained from genotypic tests were not entirely consistent with the results of the phenotypic evaluation. The genes S, A, R, and A were frequently detected in their corresponding phenotypic resistant isolates. In contrast, the genes 2, (6')-Ib, and were less frequently found in the corresponding phenotypically resistant strains. The high diversity and detection rate of ARB and ARGs in aquaculture might be a significant threat to the food chains closely related to human health.
本研究旨在深入了解中国浙江水产品中抗生素耐药菌（ARB）和抗生素耐药基因（ARGs）的分布和多样性。从六个水样中采集的 136 株疑似 ARB 通过 16S rDNA 测序被分为 22 属和 49 种。 spp.、 spp.、 spp.、 spp.、 spp.和 spp.占 ARB 的 80%。其中，109 株（80.15%）对至少一种抗生素表现出耐药性。大多数分离株不仅对最初选择的药物而且对一到三种其他测试药物表现出耐药性。分布在不同水产品中的 ARB 多样性显著。此外，从基因型测试获得的耐药数据与表型评估的结果并不完全一致。相应表型耐药分离株中经常检测到 S、A、R 和 A 基因。相比之下，相应表型耐药菌株中较少检测到 2、(6')-Ib 和 基因。养殖中 ARB 和 ARGs 的高多样性和检出率可能对与人类健康密切相关的食物链构成重大威胁。
