Graduate School of Engineering Science, Yokohama National University, Tokiwadai 79-5, Hodogaya-ku, Yokohama 240-8501, Japan.
Faculty of Engineering, Yokohama National University, Tokiwadai 79-5, Hodogaya-ku, Yokohama 240-8501, Japan.
Int J Biol Macromol. 2022 Jun 15;210:233-242. doi: 10.1016/j.ijbiomac.2022.05.027. Epub 2022 May 7.
In this study, the aggregation mechanism of polyion complex (PIC) particles from chitosan (CHI) and chondroitin sulfate C (CS) in phosphate-buffered saline (PBS) was analyzed, and a novel method for the fabrication of hydrogels via aggregation was developed. The PBS induced a decrease in the ζ-potential of the CS/CHI PIC particles, increase in their diameter, and aggregation in a concentration-dependent manner. The hydrogels prepared by mixing CS/CHI PIC particle dispersion and PBS showed the PIC components, with porous structure, high swelling ratio (161.4 ± 13.3%), and high storage moduli (26.2 ± 1.4 kPa). By mixing PBS with suspended adhesive cells and CS/CHI PIC particle dispersion, hydrogels with high cell-loading efficiency were successfully synthesized. The loaded cells within the hydrogels exhibited high viability, uniform distribution, and formation of cell aggregates. These results indicate that CS/CHI-based hydrogels have a potential application as three-dimensional scaffolds for cell culture in tissue engineering.
在这项研究中，分析了壳聚糖 (CHI) 和硫酸软骨素 C (CS) 的聚离子复合物 (PIC) 颗粒在磷酸盐缓冲盐水 (PBS) 中的聚集机制，并开发了一种通过聚集制备水凝胶的新方法。 PBS 导致 CS/CHI PIC 颗粒的 ζ 电位降低、粒径增加，并以浓度依赖的方式聚集。通过混合 CS/CHI PIC 颗粒分散体和 PBS 制备的水凝胶显示出 PIC 成分，具有多孔结构、高溶胀比（161.4 ± 13.3%）和高储能模量（26.2 ± 1.4 kPa）。通过将 PBS 与悬浮的粘性细胞和 CS/CHI PIC 颗粒分散体混合，成功合成了具有高细胞负载效率的水凝胶。负载在水凝胶内的细胞具有高活力、均匀分布和细胞聚集的形成。这些结果表明，基于 CS/CHI 的水凝胶有可能作为组织工程中细胞培养的三维支架应用。
