School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
Photochem Photobiol. 2022 Nov;98(6):1379-1389. doi: 10.1111/php.13643. Epub 2022 May 30.
Ultraviolet (UV), particularly UVB, is widely used in the treatment of skin diseases including psoriasis, atopic dermatitis, vitiligo, mycosis fungoides and pruritus. Recently, there has been a trend of replacing broad-band UVB (BB-UVB) units with narrow-band UVB (NB-UVB), as studies have demonstrated that NB-UVB is more efficacious in the treatment of psoriasis. The purpose of this study is to evaluate the biological effects and transcriptome changes induced by light-emitting diode-based NB-UVB (NB-UVB LED) phototherapy. Cell viability and the cell migration ability were significantly decreased posttreatment, as well as apoptosis and ROS levels were remarkably increased. NB-UVB-induced S phase arrest was observed 12 h postirradiation. Bioinformatics analysis of transcriptome sequencing data revealed that NB-UVB LED irradiation induced dose-depended changes in multiple key signaling pathways, such as PI3K and cytoskeletal-related pathways. The depolymerization of cytoskeleton induced by NB-UVB was observed 24 h posttreatment. In addition, the expression levels of cytoskeleton-related proteins FN1, ITGB4, ITGA1, RAC2 and DOCK1 decreased significantly 12 h after irradiation. Our results indicated that NB-UVB LED may serve as a novel option for the development of NB-UVB phototherapy devices.
紫外线（UV），特别是 UVB，广泛应用于治疗皮肤病，包括银屑病、特应性皮炎、白癜风、蕈样肉芽肿和瘙痒症。最近，有将宽谱 UVB（BB-UVB）装置替换为窄谱 UVB（NB-UVB）的趋势，因为研究表明 NB-UVB 在治疗银屑病方面更有效。本研究旨在评估基于发光二极管的 NB-UVB（NB-UVB LED）光疗的生物学效应和转录组变化。治疗后细胞活力和迁移能力明显降低，凋亡和 ROS 水平显著增加。照射后 12 小时观察到 NB-UVB 诱导的 S 期阻滞。转录组测序数据的生物信息学分析表明，NB-UVB LED 照射诱导多个关键信号通路（如 PI3K 和细胞骨架相关通路）的剂量依赖性变化。照射后 24 小时观察到细胞骨架的解聚。此外，NB-UVB 处理后 12 小时，细胞骨架相关蛋白 FN1、ITGB4、ITGA1、RAC2 和 DOCK1 的表达水平显著降低。我们的结果表明，NB-UVB LED 可能成为 NB-UVB 光疗设备发展的新选择。
