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用放射免疫溶血试验检测产肠毒素大肠杆菌的不耐热(LT)肠毒素:临床应用的一种改进方法

Detection of heat-labile (LT) enterotoxin of enterotoxigenic Escherichia coli by the radial immune hemolysis test: a modification for clinical use.

作者信息

Yano T, Oliveira M S, Fontes C F, de Almeida A C, de Castro A F

出版信息

Med Microbiol Immunol. 1982;171(3):171-8. doi: 10.1007/BF02123625.

DOI:10.1007/BF02123625
PMID:6761565
Abstract

An adaptation for clinical purposes of a radial immune hemolysis test for the detection of the heat-labile (LT) enterotoxin of the enterotoxigenic Escherichia coli is described. The results correlated well with those from Vero cell assay and the passive immune hemolysis test. Because the test is easy, relatively cheap, and as sensitive as other standard procedures, for LT detection, it is particularly suitable for less-equipped laboratories. Also, a large number of E. coli strains may be examined daily by this technique.

摘要

本文描述了一种用于临床目的的放射免疫溶血试验的改良方法,该试验用于检测产肠毒素大肠杆菌的不耐热(LT)肠毒素。其结果与Vero细胞试验和被动免疫溶血试验的结果高度相关。由于该试验操作简便、成本相对较低,且与其他标准方法一样灵敏,特别适合设备较少的实验室用于检测LT。此外,每天可通过该技术检测大量大肠杆菌菌株。

相似文献

1
Detection of heat-labile (LT) enterotoxin of enterotoxigenic Escherichia coli by the radial immune hemolysis test: a modification for clinical use.用放射免疫溶血试验检测产肠毒素大肠杆菌的不耐热(LT)肠毒素:临床应用的一种改进方法
Med Microbiol Immunol. 1982;171(3):171-8. doi: 10.1007/BF02123625.
2
Single radial immune hemolysis test for detection of Escherichia coli thermolabile enterotoxin.用于检测大肠杆菌不耐热肠毒素的单向放射免疫溶血试验。
J Clin Microbiol. 1981 Nov;14(5):473-8. doi: 10.1128/jcm.14.5.473-478.1981.
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Improvements in the passive immune hemolysis test for assaying enterotoxigenic Escherichia coli.用于检测产肠毒素大肠杆菌的被动免疫溶血试验的改进。
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Quantitative analysis of the production of heat-labile enterotoxin by enterotoxigenic Escherichia coli.产肠毒素大肠杆菌产生不耐热肠毒素的定量分析。
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Passive immune hemolysis for detection of heat-labile enterotoxin produced by Escherichia coli isolated from different sources.用于检测从不同来源分离的大肠杆菌产生的热不稳定肠毒素的被动免疫溶血法。
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Storage of Escherichia coli thermolabile enterotoxin on filter papers for assay by immune haemolysis reactions.将大肠杆菌不耐热肠毒素储存在滤纸上,用于通过免疫溶血反应进行检测。
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引用本文的文献

1
Storage of Escherichia coli thermolabile enterotoxin on filter papers for assay by immune haemolysis reactions.将大肠杆菌不耐热肠毒素储存在滤纸上,用于通过免疫溶血反应进行检测。
Med Microbiol Immunol. 1984;172(4):243-8. doi: 10.1007/BF02123719.
2
Assay for heat-labile Escherichia coli enterotoxin using sandwich erythroimmunoassay.采用夹心红细胞免疫测定法检测不耐热大肠杆菌肠毒素
Med Microbiol Immunol. 1987;176(2):89-97. doi: 10.1007/BF00200680.
3
GM1 erythroimmunoassay for detection and titration of Escherichia coli heat-labile enterotoxin.

本文引用的文献

1
Polymyxin B-Induced Release of Low-Molecular-Weight, Heat-Labile Enterotoxin from Escherichia coli.多黏菌素 B 诱导大肠埃希菌释放低分子量热不稳定肠毒素。
Infect Immun. 1974 Nov;10(5):1010-7. doi: 10.1128/iai.10.5.1010-1017.1974.
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Single radial immune hemolysis test for detection of Escherichia coli thermolabile enterotoxin.用于检测大肠杆菌不耐热肠毒素的单向放射免疫溶血试验。
J Clin Microbiol. 1981 Nov;14(5):473-8. doi: 10.1128/jcm.14.5.473-478.1981.
3
Value of passive immune hemolysis for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli.
用于检测和滴定大肠杆菌不耐热肠毒素的GM1红细胞免疫测定法。
J Clin Microbiol. 1986 Nov;24(5):744-8. doi: 10.1128/jcm.24.5.744-748.1986.
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Indirect haemagglutination test for the detection of thermolabile (LT) enterotoxin from Escherichia coli.用于检测大肠杆菌不耐热(LT)肠毒素的间接血凝试验。
Med Microbiol Immunol. 1986;175(4):251-60. doi: 10.1007/BF02123733.
被动免疫溶血法在检测产肠毒素大肠杆菌产生的热不稳定肠毒素中的价值
J Clin Microbiol. 1980 Dec;12(6):768-71. doi: 10.1128/jcm.12.6.768-771.1980.
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Improvements in the passive immune hemolysis test for assaying enterotoxigenic Escherichia coli.用于检测产肠毒素大肠杆菌的被动免疫溶血试验的改进。
J Clin Microbiol. 1980 Nov;12(5):714-7. doi: 10.1128/jcm.12.5.714-717.1980.
5
Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.用于检测产肠毒素大肠杆菌热不稳定肠毒素的改良埃莱克试验。
J Clin Microbiol. 1981 Jan;13(1):1-5. doi: 10.1128/jcm.13.1.1-5.1981.
6
Enterotoxigenic Escherichia coli isolated from patients with severe cholera-like disease.从患有严重霍乱样疾病的患者中分离出的产肠毒素大肠杆菌。
J Infect Dis. 1971 Apr;123(4):378-85. doi: 10.1093/infdis/123.4.378.
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The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin.猪源致病性大肠杆菌产生的两种明显不同的肠毒素之间的关系。
J Med Microbiol. 1970 Aug;3(3):387-401. doi: 10.1099/00222615-3-3-387.
8
Response of the rabbit ileal loop to cell-free products from Escherichia coli enteropathogenic for swine.兔回肠袢对猪致病性大肠杆菌无细胞产物的反应。
J Infect Dis. 1970 Feb;121(2):182-7. doi: 10.1093/infdis/121.2.182.
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Neutralization of cholera enterotoxin-induced steroidogenesis by specific antibody.特异性抗体对霍乱肠毒素诱导的类固醇生成的中和作用。
J Infect Dis. 1974 Mar;129(3):284-8. doi: 10.1093/infdis/129.3.284.
10
Cross-reactivity between heat labile enterotoxins of Vibrio cholerae and Escherichia coli in neutralization tests in rabbit ileum and skin.霍乱弧菌与大肠杆菌不耐热肠毒素在兔回肠和皮肤中和试验中的交叉反应性
Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Dec;81(6):757-62. doi: 10.1111/j.1699-0463.1973.tb02272.x.