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用于检测产肠毒素大肠杆菌热不稳定肠毒素的改良埃莱克试验。

Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.

作者信息

Honda T, Taga S, Takeda Y, Miwatani T

出版信息

J Clin Microbiol. 1981 Jan;13(1):1-5. doi: 10.1128/jcm.13.1.1-5.1981.

DOI:10.1128/jcm.13.1.1-5.1981
PMID:6780599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273710/
Abstract

The Elek test was modified for detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli. A total of 164 strains of E. coli were tested by the modified Elek test, and the results correlated well with those of the Chinese hamster ovary cell assay and passive immune hemolysis. It is concluded that the modified Elek test is a simple and reproducible assay method for identification of E. coli which produce heat-labile enterotoxin, and is suitable for use in clinical laboratories.

摘要

改良Elek试验用于检测产肠毒素大肠杆菌的不耐热肠毒素。用改良Elek试验对164株大肠杆菌进行检测,结果与中国仓鼠卵巢细胞试验及被动免疫溶血试验的结果相关性良好。结论:改良Elek试验是一种简单且可重复的鉴定产不耐热肠毒素大肠杆菌的检测方法,适用于临床实验室。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88e0/273710/3a1962af754a/jcm00162-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88e0/273710/3a1962af754a/jcm00162-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88e0/273710/3a1962af754a/jcm00162-0027-a.jpg

相似文献

1
Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.用于检测产肠毒素大肠杆菌热不稳定肠毒素的改良埃莱克试验。
J Clin Microbiol. 1981 Jan;13(1):1-5. doi: 10.1128/jcm.13.1.1-5.1981.
2
Further evaluation of the Biken test (modified Elek test) for detection of enterotoxigenic Escherichia coli producing heat-labile enterotoxin and application of the test to sampling of heat-stable enterotoxin.用于检测产热不稳定肠毒素的产肠毒素性大肠杆菌的比肯试验(改良埃莱克试验)的进一步评估以及该试验在热稳定肠毒素采样中的应用。
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3
Value of passive immune hemolysis for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli.被动免疫溶血法在检测产肠毒素大肠杆菌产生的热不稳定肠毒素中的价值
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Evaluation of three simple and rapid immunological tests for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.用于检测产肠毒素大肠杆菌热不稳定肠毒素的三种简单快速免疫检测方法的评估。
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A simple assay to detect Escherichia coli producing heat labile enterotoxin: results of a field study of the Biken tests in Bangladesh.一种检测产热不稳定肠毒素大肠杆菌的简易检测方法:孟加拉国比肯试验的现场研究结果
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Application of Biken test (modified Elek test) for sampling of heat-stable enterotoxin of Escherichia coli isolated in Bangladesh.运用比肯试验(改良埃莱克试验)对在孟加拉国分离出的大肠杆菌耐热肠毒素进行采样。
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[Modification of a method of passive immune hemolysis on a solid medium for detecting the production of thermolabile enterotoxins by Vibrio cholerae and Escherichia coli strains].[一种用于检测霍乱弧菌和大肠杆菌菌株产生不耐热肠毒素的固体培养基上被动免疫溶血方法的改进]
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Passive immune hemolysis for detection of heat-labile enterotoxin produced by Escherichia coli isolated from different sources.用于检测从不同来源分离的大肠杆菌产生的热不稳定肠毒素的被动免疫溶血法。
Infect Immun. 1979 Jun;24(3):606-10. doi: 10.1128/iai.24.3.606-610.1979.

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Alterations in Pseudomonas aeruginosa exoproducts by sub-MICs of some antibiotics.某些抗生素亚抑菌浓度对铜绿假单胞菌外毒素产物的影响。
Folia Microbiol (Praha). 1993;38(4):349-52. doi: 10.1007/BF02898607.
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Colorimetric detection of heat-labile toxin-encoding gene of enterotoxigenic Escherichia coli by PCR.通过聚合酶链反应比色法检测产肠毒素大肠杆菌的热不稳定毒素编码基因。

本文引用的文献

1
The recognition of toxicogenic bacterial strains in vitro.体外产毒细菌菌株的识别。
Br Med J. 1948 Mar 13;1(4549):493-6. doi: 10.1136/bmj.1.4549.493.
2
Immunological methods for detection of Kanagawa phenomenon of Vibrio parahaemolyticus.检测副溶血性弧菌神奈川现象的免疫学方法。
J Clin Microbiol. 1980 Jun;11(6):600-3. doi: 10.1128/jcm.11.6.600-603.1980.
3
Value of passive immune hemolysis for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli.被动免疫溶血法在检测产肠毒素大肠杆菌产生的热不稳定肠毒素中的价值
J Clin Microbiol. 1995 Jul;33(7):1957-60. doi: 10.1128/jcm.33.7.1957-1960.1995.
4
In vitro formation of hybrid toxins between subunits of Escherichia coli heat-labile enterotoxin and those of cholera enterotoxin.大肠杆菌不耐热肠毒素亚基与霍乱肠毒素亚基之间杂合毒素的体外形成。
Infect Immun. 1981 Nov;34(2):341-6. doi: 10.1128/iai.34.2.341-346.1981.
5
Production of cholera-like enterotoxin by a Vibrio cholerae non-O1 strain isolated from the environment.从环境中分离出的一株非O1群霍乱弧菌产生霍乱样肠毒素。
Infect Immun. 1981 Oct;34(1):90-7. doi: 10.1128/iai.34.1.90-97.1981.
6
Detection of heat-labile (LT) enterotoxin of enterotoxigenic Escherichia coli by the radial immune hemolysis test: a modification for clinical use.用放射免疫溶血试验检测产肠毒素大肠杆菌的不耐热(LT)肠毒素:临床应用的一种改进方法
Med Microbiol Immunol. 1982;171(3):171-8. doi: 10.1007/BF02123625.
7
Evaluation of a defined medium for the production of both thermolabile (LT) and thermostable (ST) enterotoxins of Escherichia coli.用于生产大肠杆菌不耐热肠毒素(LT)和耐热肠毒素(ST)的特定培养基的评估。
Med Microbiol Immunol. 1982;171(1):43-51. doi: 10.1007/BF02122706.
8
Rapid visual detection of Escherichia coli and Vibrio cholerae Heat-labile enterotoxins by nitrocellulose enzyme-linked immunosorbent assay.通过硝酸纤维素酶联免疫吸附测定法快速视觉检测大肠杆菌和霍乱弧菌不耐热肠毒素。
J Clin Microbiol. 1984 Mar;19(3):371-5. doi: 10.1128/jcm.19.3.371-375.1984.
9
Improved GM1-enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.用于检测大肠杆菌不耐热肠毒素的改良GM1酶联免疫吸附测定法。
J Clin Microbiol. 1983 Oct;18(4):808-15. doi: 10.1128/jcm.18.4.808-815.1983.
10
Identification of enterotoxigenic Escherichia coli in patients with diarrhea in Asia with three enterotoxin gene probes.用三种肠毒素基因探针鉴定亚洲腹泻患者中的产肠毒素大肠杆菌。
Infect Immun. 1983 Oct;42(1):152-5. doi: 10.1128/iai.42.1.152-155.1983.
J Clin Microbiol. 1980 Dec;12(6):768-71. doi: 10.1128/jcm.12.6.768-771.1980.
4
Enterotoxigenic Escherichia coli isolated from patients with severe cholera-like disease.从患有严重霍乱样疾病的患者中分离出的产肠毒素大肠杆菌。
J Infect Dis. 1971 Apr;123(4):378-85. doi: 10.1093/infdis/123.4.378.
5
The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin.猪源致病性大肠杆菌产生的两种明显不同的肠毒素之间的关系。
J Med Microbiol. 1970 Aug;3(3):387-401. doi: 10.1099/00222615-3-3-387.
6
Response of the rabbit ileal loop to cell-free products from Escherichia coli enteropathogenic for swine.兔回肠袢对猪致病性大肠杆菌无细胞产物的反应。
J Infect Dis. 1970 Feb;121(2):182-7. doi: 10.1093/infdis/121.2.182.
7
Neutralization of cholera enterotoxin-induced steroidogenesis by specific antibody.特异性抗体对霍乱肠毒素诱导的类固醇生成的中和作用。
J Infect Dis. 1974 Mar;129(3):284-8. doi: 10.1093/infdis/129.3.284.
8
Cross-reactivity between heat labile enterotoxins of Vibrio cholerae and Escherichia coli in neutralization tests in rabbit ileum and skin.霍乱弧菌与大肠杆菌不耐热肠毒素在兔回肠和皮肤中和试验中的交叉反应性
Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Dec;81(6):757-62. doi: 10.1111/j.1699-0463.1973.tb02272.x.
9
Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture.利用组织培养中的肾上腺细胞检测不耐热大肠杆菌肠毒素。
Science. 1974 Jan 25;183(4122):334-6. doi: 10.1126/science.183.4122.334.
10
Identification of enterotoxigenic Escherichia coli and serum antitoxin activity by the vascular permeability factor assay.通过血管通透性因子测定法鉴定产肠毒素大肠杆菌及血清抗毒素活性
Infect Immun. 1973 Nov;8(5):731-5. doi: 10.1128/iai.8.5.731-735.1973.