Engman D M, Reddy L V, Donelson J E, Kirchhoff L V
Mol Biochem Parasitol. 1987 Jan 15;22(2-3):115-23. doi: 10.1016/0166-6851(87)90041-7.
Molecular karyotypes of 6 strains and 6 clones of Trypanosoma cruzi were determined using orthogonal-field-alternation gel electrophoresis. At least 15 different chromosome-sized DNA molecules, ranging in size from less than 200 kilobase pairs to greater than 2000 kilobase pairs, were resolved for each of the isolates examined. Many of the bands were present in different relative intensities suggesting that the number of individual chromosomes per organism may be considerably higher. Significant inter- and intra-strain differences in molecular karyotype and in the chromosomal locations of the genes for the spliced leader, tubulins, 5S ribosomal RNA and a heat shock protein were found. These marked chromosomal differences among T. cruzi strains and clones may be related to the high degree of phenotypic heterogeneity previously found in this parasite.
利用正交场交变凝胶电泳测定了6株克氏锥虫和6个克隆的分子核型。对于所检测的每一个分离株，至少分辨出15种不同染色体大小的DNA分子，其大小范围从小于200千碱基对到大于2000千碱基对。许多条带以不同的相对强度出现，这表明每个生物体中单个染色体的数量可能要高得多。在分子核型以及剪接前导序列、微管蛋白、5S核糖体RNA和一种热休克蛋白的基因的染色体位置方面，发现了显著的株间和株内差异。克氏锥虫不同菌株和克隆之间这些明显的染色体差异可能与此前在这种寄生虫中发现的高度表型异质性有关。
