Cincotto Fernando H, Carvalho Daniel A S, Canevari Thiago C, Toma Henrique E, Fatibello-Filho Orlando, Moraes Fernando C
Chemistry Department, Federal University of São Carlos 13565-905 São Carlos SP Brazil
Engineering School, Mackenzie Presbyterian University 01302-907 São Paulo SP Brazil.
RSC Adv. 2018 Apr 16;8(25):14040-14047. doi: 10.1039/c8ra01857j. eCollection 2018 Apr 11.
The simultaneous electrochemical detection of mood disorder related substances, such as amitriptyline, melatonin and tryptophan, was successfully achieved by using a novel nano-magnetic electrochemical sensor design, encompassing FeO nanoparticles decorated with carbon quantum dots (MagNPs/Cdots). The magnetic composite was characterized using HR-TEM microscopy, XRD and Raman spectroscopy, and was applied onto a glassy carbon electrode using a miniature neodymium magnet. The determination of amitriptyline, melatonin and tryptophan was performed by monitoring oxidation promoted by MagNPs/Cdots in BR-buffer at pH 3.0, which proceeded according to well-defined differential pulse voltammetry peaks, with detection limits of 5.9, 4.4 and 4.2 nmol L, respectively. No significant interference was seen from biological interferents such as uric acid, ascorbic acid, dopamine, estriol and 17β-estradiol. The magnetic hybrid material was highly stable in solution, opening exciting opportunities for the development of low cost and practical electrochemical sensors for the determination of mood disorder related substances in real clinical samples.
通过使用一种新型的纳米磁性电化学传感器设计，成功实现了对与情绪障碍相关物质（如阿米替林、褪黑素和色氨酸）的同时电化学检测，该设计包括用碳量子点修饰的FeO纳米颗粒（MagNPs/Cdots）。使用高分辨率透射电子显微镜（HR-TEM）、X射线衍射（XRD）和拉曼光谱对磁性复合材料进行了表征，并使用微型钕磁铁将其应用于玻碳电极上。通过监测在pH 3.0的BR缓冲液中由MagNPs/Cdots促进的氧化反应来测定阿米替林、褪黑素和色氨酸，该氧化反应按照明确的差分脉冲伏安法峰进行，检测限分别为5.9、4.4和4.2 nmol/L。尿酸、抗坏血酸、多巴胺、雌三醇和17β-雌二醇等生物干扰物未产生明显干扰。磁性杂化材料在溶液中高度稳定，为开发低成本、实用的电化学传感器以测定实际临床样品中与情绪障碍相关的物质提供了令人兴奋的机会。
