Shastan Z Omara, Ganesh Hashwin V S, Noroozifar Meissam, Kerman Kagan
Department of Chemistry, University of Sistan and Baluchestan P. O. Box 98135-674 Zahedan Iran
Department of Physical and Environmental Sciences, University of Toronto Scarborough 1265 Military Trail Toronto Ontario M1C 1A4 Canada
RSC Adv. 2018 Feb 6;8(11):5960-5966. doi: 10.1039/c7ra09603h. eCollection 2018 Feb 2.
In this report, simultaneous electrochemical determination of ascorbic acid (AA), dopamine (DA), uric acid (UA) and tryptophan (Trp) was achieved using buckyball-modified carbon ceramic microelectrodes (CCMEs). A concentration-dependent increase in anodic peak current signals was observed in comparison with those obtained at bare CCMEs. The optimal pH for simultaneous determination of a quaternary mixture of AA-DA-UA-Trp was determined to be pH 4. The peak separations for the mixture containing AA-DA-UA-Trp were well-defined at the scan rate of 50 mV s. The catalytic peak current obtained was linearly dependent on the AA, DA, UA and Trp concentrations in the range of 6.0-600, 6.0-600, 6.0-600 and 4.0-440 μM, respectively. The detection limits for AA, DA, UA and Trp were also determined to be 1.64, 0.82, 0.36 and 1.22 μM, respectively. The analytical performance of this sensor has also been challenged for simultaneous electrochemical detection of AA, DA, UA and Trp in real samples.
在本报告中，使用巴基球修饰的碳陶瓷微电极（CCMEs）实现了同时电化学测定抗坏血酸（AA）、多巴胺（DA）、尿酸（UA）和色氨酸（Trp）。与在裸CCMEs上获得的信号相比，观察到阳极峰值电流信号呈浓度依赖性增加。测定AA-DA-UA-Trp四元混合物的最佳pH值为pH 4。在50 mV s的扫描速率下，含有AA-DA-UA-Trp的混合物的峰分离良好。获得的催化峰值电流分别在6.0 - 600、6.0 - 600、6.0 - 600和4.0 - 440 μM范围内与AA、DA、UA和Trp浓度呈线性相关。AA、DA、UA和Trp的检测限也分别确定为1.64、0.82、0.36和1.22 μM。该传感器的分析性能也受到了在实际样品中同时电化学检测AA、DA、UA和Trp的挑战。
