一种使用富勒烯探针检测尿素的简便且高灵敏度的共振瑞利散射-能量转移方法。
A facile and highly sensitive resonance Rayleigh scattering-energy transfer method for urea using a fullerene probe.
作者信息
Yao Dongmei, He Zining, Wen Guiqing, Liang Aihui, Jiang Zhiliang
机构信息
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology Guilin 541004 China
College of Chemistry and Biology Engineering, Hechi University Yizhou 546300 China.
出版信息
RSC Adv. 2018 Aug 14;8(51):29008-29012. doi: 10.1039/c8ra05269g.
Under ultrasound conditions, a deep yellow fullerene (C) colloid was prepared, which exhibits two resonance Rayleigh scattering peaks at 385 nm and 530 nm. Urea was reacted with dimethylglyoxime (DMG) to produce 4,5-dimethyl-2-imidazole ketone (DIK), in the presence of stabilizer thiosemicarbazone (TSC). Resonance Rayleigh scattering energy transfer (RRS-ET) was shown to occur between the donor fullerene and acceptor DIK due to an overlap of the DIK absorption and fullerene RRS peaks. Upon an increase in the urea concentration, the RRS-ET was enhanced and the RRS intensity decreased. The decreased RRS intensity was linear to the urea concentration in the range of 6.66-333.00 nmoL L, with a detection limit of 2.0 nmoL L. Accordingly, a new and simple RRS-ET method was established for detecting trace levels of urea in foods, with satisfactory results.
在超声条件下，制备了一种深黄色的富勒烯（C）胶体，其在385nm和530nm处呈现两个共振瑞利散射峰。尿素与丁二酮肟（DMG）反应生成4,5-二甲基-2-咪唑酮（DIK），反应过程中存在稳定剂硫代卡巴腙（TSC）。由于DIK吸收峰与富勒烯共振瑞利散射峰的重叠，表明供体富勒烯与受体DIK之间发生了共振瑞利散射能量转移（RRS-ET）。随着尿素浓度的增加，RRS-ET增强，共振瑞利散射强度降低。在6.66 - 333.00 nmoL/L范围内，共振瑞利散射强度的降低与尿素浓度呈线性关系，检测限为2.0 nmoL/L。据此，建立了一种新颖且简便的RRS-ET方法用于检测食品中的痕量尿素，结果令人满意。
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