Ccorahua Robert, Moreno Silvia, Gumz Hannes, Sahre Karin, Voit Brigitte, Appelhans Dietmar
Department of Mechanical Engineering, Pontificia Universidad Católica del Peru Lima 32 Peru.
Leibniz-Institut für Polymerforschung Dresden e.V. Hohe Straße 6 01069 Dresden Germany
RSC Adv. 2018 Jul 17;8(45):25436-25443. doi: 10.1039/c8ra03964j. eCollection 2018 Jul 16.
Reconstitution of biologically active polymersomes from the frozen or solid state into any fluid state is still a challenging issue for the design of new biological experiments and for the formulation of therapeutic agents. To gain knowledge about the reconstitution of pH-responsive and photo-crosslinked polymersomes, surface-functionalized and enzyme-containing polymersomers were cryogenically frozen (-20 °C) or freeze-dried with inulin as the lyoprotectant (0.1% w/v) and stored for a defined time period. Reconstituting those polymersomes in solution by thawing or a re-dispersing process revealed their original physical properties as well as their function as a pH-switchable enzymatic nanoreactor.
将冷冻或固态的生物活性聚合物囊泡重构为任何流体状态，对于新生物实验的设计和治疗剂的配方而言，仍然是一个具有挑战性的问题。为了了解pH响应性和光交联聚合物囊泡的重构情况，将表面功能化且含酶的聚合物囊泡在-20°C下低温冷冻，或用菊粉作为冻干保护剂（0.1% w/v）进行冻干，并储存一段规定的时间。通过解冻或再分散过程在溶液中重构这些聚合物囊泡，揭示了它们原有的物理性质以及作为pH可切换酶促纳米反应器的功能。
