Laboratoire de Chimie des Polymères Organiques, LCPO, Université de Bordeaux , CNRS, Bordeaux INP, UMR 5629, 16 Avenue Pey Berland F-33600 Pessac, France.
Langmuir. 2017 Jul 18;33(28):7079-7085. doi: 10.1021/acs.langmuir.7b00655. Epub 2017 Jul 6.
Multicompartmentalization is a key feature of eukaryotic cells, allowing separation and protection of species within the membrane walls. During the last years, several methods have been reported to afford synthetic multicompartment lipidic or polymeric vesicles that mimic biological cells and that allow cascade chemical or enzymatic reactions within their lumen. We hereby report on the preparation and study of liposomes in polymersomes (LiPs) systems. We discuss on the loading and coloading of lipidic nanovesicles made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipentadecanoyl-sn-glycero-3-phosphocholine (diC15-PC), or 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) inside the lumen of giant poly(butadiene)-b-poly(ethylene oxide) (PBut-b-PEO) polymersomes. These LiPs systems were characterized by confocal microscopy and UV-visible spectroscopy. We further demonstrate that we can achieve controlled sequential release of dyes from diC15-PC and DPPC liposomes at defined temperatures inside the giant PBut-b-PEO polymersomes. This controlled release could be used as a means to initiate cascade reactions on demand in confined microreactors.
多隔间化是真核细胞的一个关键特征，允许在膜壁内分离和保护物种。在过去的几年中，已经报道了几种方法来提供模拟生物细胞的合成多隔间脂质体或聚合物囊泡，并且允许在其内腔中进行级联化学或酶反应。在此，我们报告了在聚合物囊泡（LiPs）系统中制备和研究脂质体的情况。我们讨论了装载和共装载由 1-棕榈酰基-2-油酰基-sn-甘油-3-磷酸胆碱（POPC）、1,2-二肉豆蔻酰基-sn-甘油-3-磷酸胆碱（DMPC）、1,2-二十五烷酰基-sn-甘油-3-磷酸胆碱（diC15-PC）或 1,2-二棕榈酰基-sn-甘油-3-磷酸胆碱（DPPC）制成的脂质纳米囊泡在巨大的聚丁二烯-b-聚氧化乙烯（PBut-b-PEO）聚合物囊泡内腔中的情况。这些 LiPs 系统通过共聚焦显微镜和紫外可见光谱进行了表征。我们进一步证明，我们可以在巨大的 PBut-b-PEO 聚合物囊泡内的定义温度下从 diC15-PC 和 DPPC 脂质体中实现受控顺序释放染料。这种受控释放可以用作按需在封闭微反应器中引发级联反应的手段。
