Yang Xue, Ma Ke, Yang Libo, Chen Yujuan, Qu Yingmin, Wang Ying, Wang Xinyue, Yang Fan, Sun Qi, Song Zhengxun, Wang Zuobin
International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology Changchun 130022 China
School of Electronic Information Engineering, Changchun University of Science and Technology Changchun 130022 China
RSC Adv. 2019 Dec 11;9(70):40887-40894. doi: 10.1039/c9ra07180f. eCollection 2019 Dec 9.
This paper employs a spin-coated method to construct conductive polypyrrole (PPy) substrates which present superior properties for controlling the morphological structures and functions of bEnd.3 cells. The PPy substrates with a homogeneous particle size, uniform distribution and proper roughness show enhanced hydrophilic characteristics and improve cell adhesion to the substrates. The changes in the mechanical properties of cells and the responses to the designed substrates and magnetic field are also explored. Due to the synergistic effect between the magnetic field and the conductive PPy substrate, the cells cultured in such an environment exhibit applanate shapes with more branches and enhanced cell viability. In addition, the cells preferentially extend along the magnetic field direction. The mechanical characteristics of cells change significantly under varying magnetic intensity stimulations (5-16 mT). The satisfying effect on cells' morphology and outgrowth is acquired at the magnetic intensities of 9-10 mT and duration of 20 min, compared with other stimulated groups, while retaining cell viability. Moreover, the cells express higher adhesion up to 5.2 nN. The results suggest that the application of the PPy substrates and magnetic field is a promising candidate for the protection of neurovascular units and treatment of neurological diseases.
本文采用旋涂法构建导电聚吡咯（PPy）基底，该基底在控制bEnd.3细胞的形态结构和功能方面具有优异性能。具有均匀粒径、均匀分布和适当粗糙度的PPy基底表现出增强的亲水性特征，并改善了细胞对基底的粘附。还探索了细胞力学性能的变化以及对设计基底和磁场的响应。由于磁场与导电PPy基底之间的协同效应，在这种环境中培养的细胞呈现出扁平形状，具有更多分支且细胞活力增强。此外，细胞优先沿磁场方向延伸。在不同磁场强度刺激（5 - 16 mT）下，细胞的力学特性发生显著变化。与其他刺激组相比，在9 - 10 mT的磁场强度和20分钟的持续时间下，对细胞形态和生长的影响令人满意，同时保持细胞活力。此外，细胞表现出高达5.2 nN的更高粘附力。结果表明，PPy基底和磁场的应用有望用于保护神经血管单元和治疗神经系统疾病。
