Cheng Jun, Lu Hongxiang, Li Ke, Zhu Yanxia, Zhou Junhu
State Key Laboratory of Clean Energy Utilization, Zhejiang University Hangzhou 310027 China.
RSC Adv. 2018 May 8;8(30):16824-16833. doi: 10.1039/c8ra01626g. eCollection 2018 May 3.
The biomass yield of mutant ZJU9000 was 176% higher than that of wild type on day 4, and the results of transcriptome sequencing showed that processes related to cell growth were synergistically enhanced in this mutant. The amount of energy for biomass accumulation increased because the efficiency of the photoreaction was enhanced by the elevated levels of chlorophyll and carotene. The increased biosynthesis rates of ribose phosphate, nucleotides and multiple vitamins increased the production of genetic materials for cell proliferation. Furthermore, the carbon concentration mechanism in mutant ZJU9000 was enhanced, indicating the increased utilization efficiency of CO at low concentration (0.04 vol% in air). The enhancement of these growth-relevant metabolic pathways contributed to the robust growth of mutant ZJU9000.
在第4天，突变体ZJU9000的生物量产量比野生型高176%，转录组测序结果表明，该突变体中与细胞生长相关的过程协同增强。由于叶绿素和胡萝卜素水平的提高增强了光反应效率，生物量积累的能量增加。磷酸核糖、核苷酸和多种维生素生物合成速率的提高增加了细胞增殖的遗传物质产量。此外，突变体ZJU9000的碳浓缩机制增强，表明其在低浓度CO（空气中0.04体积%）下的利用效率提高。这些与生长相关的代谢途径的增强促进了突变体ZJU9000的强劲生长。
