Liu Menglong, Wang Ying, Hu Xiaodong, He Weifeng, Gong Yali, Hu Xiaohong, Liu Meixi, Luo Gaoxing, Xing Malcolm, Wu Jun
Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, Third Military Medical University (Army Medical University) Chongqing 400038 China
State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University Chengdu 610065 China.
RSC Adv. 2018 Jul 25;8(47):26626-26639. doi: 10.1039/c8ra03234c. eCollection 2018 Jul 24.
The requirements for anti-permeation, anti-infection and antifouling when treating a malicious wound bed raise new challenges for wound dressing. The present study used ,-dimethylformamide to treat poly(vinylidene fluoride) (PVDF) in order to obtain a dressing impregnated with generated nano-silver particles (NS) an immersion phase inversion method. Scanning electron microscopy (SEM) images showed that the film was characterized by a two-layer asymmetric structure with different pore sizes (top layer: ∼0.4 μm; bottom layer: ∼1.8 μm). The moisture permeability test indicated that the film had an optimal water vapor transmission rate (WVTR: ∼2500 g m per day). TEM images revealed the successful formation of spherical NS, and Fourier-transform infrared spectroscopy (FTIR) demonstrated the integration of PVDF and NS (, PVDF/NS). Correspondingly, the water contact angle measurements confirmed increased membrane surface hydrophobicity after NS integration. The inductively coupled plasma (ICP) spectrometry showed that the PVDF/NS displayed a continuous and safe release of silver ions. Moreover, experiments indicated that PVDF/NS films possessed satisfactory anti-permeation, antibacterial and antifouling activities against and bacteria, while they exhibited no obvious cytotoxicity toward mammalian HaCaT cells. Finally, the results showed that the nanoporous top layer of film could serve as a physical barrier to prevent bacterial penetration, whereas the microporous bottom layer could efficiently prevent bacterial infection caused by biofouling, leading to fast re-epithelialization the enhancement of keratinocyte proliferation. Collectively, the results show that the PVDF/NS25 film has a promising application in wound treatment, especially for wounds infected by multi-drug-resistant bacteria such as .
处理恶性伤口床时对防渗透、抗感染和防污的要求给伤口敷料带来了新的挑战。本研究使用N，N-二甲基甲酰胺处理聚偏氟乙烯（PVDF），以便通过浸没相转化法获得浸渍有生成的纳米银颗粒（NS）的敷料。扫描电子显微镜（SEM）图像显示，该膜具有两层不对称结构，孔径不同（顶层：约0.4μm；底层：约1.8μm）。透湿性测试表明，该膜具有最佳的水蒸气透过率（WVTR：约2500g/m²·天）。透射电子显微镜（TEM）图像显示成功形成了球形NS，傅里叶变换红外光谱（FTIR）证明了PVDF和NS的结合（即PVDF/NS）。相应地，水接触角测量证实了NS结合后膜表面疏水性增加。电感耦合等离子体（ICP）光谱表明，PVDF/NS显示出银离子的持续安全释放。此外，实验表明，PVDF/NS膜对金黄色葡萄球菌和大肠杆菌具有令人满意的防渗透、抗菌和防污活性，而对哺乳动物HaCaT细胞没有明显的细胞毒性。最后，体内结果表明，膜的纳米多孔顶层可作为物理屏障防止细菌渗透，而微孔底层可有效防止生物污垢引起的细菌感染，从而导致快速上皮化并增强角质形成细胞增殖。总体而言，结果表明PVDF/NS25膜在伤口治疗中具有广阔的应用前景，特别是对于由耐多药细菌如金黄色葡萄球菌感染的伤口。
