基于 MA 改性细菌纤维素膜的纳米 ZnO/BCM 生物复合材料的原位制备及其抗菌和创伤愈合性能。

In situ Fabrication of Nano ZnO/BCM Biocomposite Based on MA Modified Bacterial Cellulose Membrane for Antibacterial and Wound Healing.

机构信息

Department of Plastic Surgery, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University), Chongqing 400038, People's Republic of China.

Engineering Research Center in Biomaterials, Sichuan University, Chengdu 610065, People's Republic of China.

出版信息

Int J Nanomedicine. 2020 Jan 6;15:1-15. doi: 10.2147/IJN.S231556. eCollection 2020.

DOI:10.2147/IJN.S231556

PMID:32021161

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6954087/

Abstract

BACKGROUND

Developing an ideal wound dressing that meets the multiple demands of safe and practical, good biocompatibility, superior mechanical property and excellent antibacterial activity is highly desirable for wound healing. Bacterial cellulose (BC) is one of such promising class of biopolymers since it can control wound exudates and can provide moist environment to a wound resulting in better wound healing. However, the lack of antibacterial activity has limited its application.

METHODS AND RESULTS

We prepared a flexible dressing based on a bacterial cellulose membrane and then modified it by chemical crosslinking to prepare in situ synthesis of nZnO/BCM via a facile and eco-friendly approach. Scanning electron microscopy (SEM) results indicated that nZnO/BCM membranes were characterized by an ideal porous structure (pore size: 30~ 90 μm), forming a unique string-beaded morphology. The average water vapor transmission of nZnO/BCM was 2856.60 g/m/day, which improved the moist environment of nZnO/BCM. ATR-FITR further confirmed the stepwise deposition of nano-zinc oxide. Tensile testing indicated that our nanocomposites were flexible, comfortable and resilient. Bacterial suspension assay and plate counting methods demonstrated that 5wt. % nZnO/BCM possessed excellent antibacterial activity against and , while MTT assay demonstrated that they had no measurable cytotoxicity toward mammalian cells. Moreover, skin irritation test and histocompatibility examination supported that 5wt. % nZnO/BCM had no stimulation to skin and had acceptable biocompatibility with little infiltration of the inflammatory cells. Finally, by using a bacteria-infected ( and ) murine wound model, we found that nZnO/BCM could prevent in vivo bacterial infections and promote wound healing via accelerating the re-epithelialization and wound contraction, and these membranes had no obvious toxicity toward normal tissues.

CONCLUSION

Therefore, the constructed nZnO/BCM has great potential for biomedical applications as an efficient antibacterial wound dressing.

摘要

背景

开发一种理想的伤口敷料，满足安全实用、良好的生物相容性、优异的机械性能和卓越的抗菌活性等多种需求，对于伤口愈合非常理想。细菌纤维素 (BC) 是一种很有前途的生物聚合物，因为它可以控制伤口渗出物，并为伤口提供湿润的环境，从而促进伤口愈合。然而，缺乏抗菌活性限制了它的应用。

方法和结果

我们制备了一种基于细菌纤维素膜的柔性敷料，然后通过化学交联对其进行修饰，通过简便、环保的方法制备原位合成 nZnO/BCM。扫描电子显微镜 (SEM) 结果表明，nZnO/BCM 膜具有理想的多孔结构（孔径：30~90μm），形成独特的串珠状形态。nZnO/BCM 的平均水蒸气透过率为 2856.60 g/m/day，提高了 nZnO/BCM 的湿润环境。ATR-FITR 进一步证实了纳米氧化锌的逐步沉积。拉伸试验表明，我们的纳米复合材料具有柔韧性、舒适性和弹性。细菌悬浮液试验和平板计数法表明，5wt.% nZnO/BCM 对和具有优异的抗菌活性，而 MTT 试验表明它们对哺乳动物细胞没有可测量的细胞毒性。此外，皮肤刺激试验和组织相容性检查表明，5wt.% nZnO/BCM 对皮肤无刺激，与皮肤的生物相容性好，炎症细胞浸润少。最后，通过使用细菌感染（和）的小鼠伤口模型，我们发现 nZnO/BCM 可以通过促进再上皮化和伤口收缩来防止体内细菌感染，并促进伤口愈合，这些膜对正常组织没有明显的毒性。