Zhuang Luer, Zhao Ling, Yin Pinghe
Department of Environmental Engineering, Jinan University Guangzhou 510632 China
Research Center of Analytical Testing, Jinan University Guangzhou 510632 China
RSC Adv. 2018 Apr 3;8(23):12760-12766. doi: 10.1039/c8ra00749g.
The algicidal compounds produced by sp. strain B1 against , one of the main red-tide algae, were isolated and identified in a previous study as urocanic acid (uro), l-histidine (his) and -acetylhistamine (ace). The 96 h median effective concentration EC values indicated the algicidal effect order of uro (8 μg mL) > ace (16 μg mL) > his (23 μg mL). The interaction between uro and ace had a synergistic effect on , accelerated the increase in its intracellular reactive oxygen species (ROS) levels, and further decreased the activities of antioxidases after 96 h, causing destruction of cell membrane integrity and nuclear structure. However, the other two binary mixtures uro + his and ace + his were both antagonistic to . The increase in the level of ROS indicated that the algal cells suffered from oxidative damage. The surplus ROS induced the increase in malondialdehyde (MDA) content and activities of antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT), all of which reached maxima after 72 h treatment. Transmission electron microscopy (TEM) analysis revealed that these nitrogen-containing compounds caused destruction of cell membrane integrity, chloroplasts and nuclear structure. The present study will provide useful information for the combined effect of algicidal compounds on the harmful alga . This is the first report to explore single and combined algicidal effects of three nitrogen-containing compounds against the harmful alga .
在之前的一项研究中，从菌株B1产生的对主要赤潮藻类之一的杀藻化合物中分离并鉴定出了尿刊酸（uro）、L-组氨酸（his）和N-乙酰组胺（ace）。96小时半数有效浓度（EC）值表明杀藻效果顺序为uro（8μg/mL）>ace（16μg/mL）>his（23μg/mL）。uro和ace之间的相互作用对该藻类具有协同效应，加速了其细胞内活性氧（ROS）水平的升高，并在96小时后进一步降低了抗氧化酶的活性，导致细胞膜完整性和核结构遭到破坏。然而，另外两种二元混合物uro + his和ace + his对该藻类均具有拮抗作用。ROS水平的升高表明藻类细胞受到了氧化损伤。过量的ROS诱导丙二醛（MDA）含量以及包括超氧化物歧化酶（SOD）和过氧化氢酶（CAT）在内的抗氧化酶活性增加，所有这些在处理72小时后均达到最大值。透射电子显微镜（TEM）分析表明，这些含氮化合物导致了细胞膜完整性、叶绿体和核结构的破坏。本研究将为杀藻化合物对有害藻类的联合作用提供有用信息。这是第一份探索三种含氮化合物对有害藻类的单一和联合杀藻作用的报告。
