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基于超高效液相色谱-电喷雾电离-四极杆-飞行时间串联质谱（UHPLC-ESI-Q-TOF-MS/MS）及多种分析策略的正常大鼠和慢性不可预测性轻度应激诱导抑郁大鼠粪便中汤剂的代谢谱分析

Metabolic profile analysis of decoction in feces of normal and chronic unpredictable mild stress-induced depression rats based on UHPLC-ESI-Q-TOF-MS/MS and multiple analytical strategies.

作者信息

Luo Kaiwen, Xing Yadong

机构信息

School of Pharmacy, Bengbu Medical University 2600 Donghai Avenue Bengbu 233030 China

出版信息

RSC Adv. 2019 Dec 5;9(68):40037-40050. doi: 10.1039/c9ra06486a. eCollection 2019 Dec 2.

DOI:10.1039/c9ra06486a
PMID:35541428
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9076205/
Abstract

decoction () has been verified by clinical application that it has definite curative effects and low side effects on depression. Because it is administered orally, the metabolites of in the intestinal tract may influence the curative effects significantly. In this study, UHPLC-ESI-Q-TOF-MS/MS was used in combination with untargeted metabolomics-driven strategy, series product ion filtering and diagnostic fragment ion strategy for acquiring the comprehensive metabolic profile of in feces of normal and chronic unpredictable mild stress (CUMS)-induced depression rats after oral administration, while the rat depression model was evaluated by behavior tests and plasma biochemical indices. Finally, a total of 56 compounds, including 35 prototype compounds and 21 metabolites, were identified or tentatively characterized in fecal samples. Among these, ten compounds were sieved as potential chemical markers that would reflect the antidepressant effect of , which may offer important information for quality assessment, pharmacokinetic study and clinical security. In conclusion, the metabolic profile of in normal and CUMS-induced depression rats would be helpful for the further study of anti-depression material basis and mechanism.

摘要

已通过临床应用验证，（某种）汤剂对抑郁症具有确切疗效且副作用低。由于其通过口服给药，（该汤剂中成分）在肠道中的代谢产物可能会对疗效产生显著影响。在本研究中，超高效液相色谱-电喷雾电离-四极杆-飞行时间串联质谱（UHPLC-ESI-Q-TOF-MS/MS）与非靶向代谢组学驱动策略、串联产物离子过滤和诊断性碎片离子策略相结合，用于获取正常大鼠和慢性不可预测轻度应激（CUMS）诱导的抑郁大鼠口服给药后粪便中（该汤剂成分）的综合代谢谱，同时通过行为测试和血浆生化指标对大鼠抑郁模型进行评估。最后，在粪便样本中总共鉴定或初步表征了56种化合物，包括35种原型化合物和21种代谢产物。其中，筛选出10种化合物作为潜在的化学标志物，它们可反映（该汤剂）的抗抑郁作用，这可能为质量评估、药代动力学研究和临床安全性提供重要信息。总之，正常大鼠和CUMS诱导的抑郁大鼠中（该汤剂成分）的代谢谱将有助于进一步研究抗抑郁物质基础和作用机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0830/9076205/348dd7c588a7/c9ra06486a-f9.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0830/9076205/348dd7c588a7/c9ra06486a-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0830/9076205/2e1e06a775e4/c9ra06486a-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0830/9076205/49e888d24104/c9ra06486a-f7.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0830/9076205/348dd7c588a7/c9ra06486a-f9.jpg

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