State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Talanta. 2011 Oct 15;85(5):2517-21. doi: 10.1016/j.talanta.2011.08.015. Epub 2011 Aug 12.
Ochratoxin A, a toxin produced by Aspergillus ochraceus and Penicillium verrucosum, is one of the most abundant food-contaminating mycotoxins in the world. It has been classified by the International Agency for Research on Cancer (IARC) as a possible human carcinogen. In this paper, a sensitive and selective fluorescent aptasensor for ochratoxin A (OTA) detection was constructed, utilizing single-walled carbon nanotubes (SWNTs) as quencher which can quench the fluorescence of free unfolded toxin-specific aptamer attached with FAM (carboxyfluorescein). Without any coating materials as compared to graphene-oxide based sensor, we obtained the detection limit of our sensing platform based on SWNTs to be 24.1 nM with a linear detection range from 25 nM to 200 nM. This technique responded specifically to OTA without interference from other analogues (N-acetyl-L-phenylalanine, warfarin and OTB). It has also been verified for real sample application by testing 1% beer containing buffer solution spiked with a series of concentration of OTA.
赭曲霉毒素 A 是由赭曲霉和疣孢青霉产生的一种毒素，是世界上最丰富的食物污染霉菌毒素之一。国际癌症研究机构 (IARC) 将其归类为可能的人类致癌物。在本文中，我们构建了一种用于赭曲霉毒素 A (OTA) 检测的灵敏和选择性荧光适体传感器，利用单壁碳纳米管 (SWNTs) 作为猝灭剂，可猝灭与 FAM（羧基荧光素）相连的未折叠游离毒素特异性适体的荧光。与基于氧化石墨烯的传感器相比，我们没有使用任何涂层材料，基于 SWNTs 的传感平台的检测限为 24.1 nM，线性检测范围为 25 nM 至 200 nM。该技术对 OTA 具有特异性响应，不受其他类似物（N-乙酰-L-苯丙氨酸、华法林和 OTB）的干扰。它还通过测试含有缓冲液的 1%啤酒中一系列 OTA 浓度的加标溶液，验证了其在实际样品应用中的可行性。
