Qi Shaolong, Zhu Lubao, Wang Xinyu, Du Jianshi, Yang Qingbiao, Li Yaoxian
China-Japan Union Hospital of Jilin University, Key Laboratory of Lymphatic Surgery Jilin Province, Engineering Laboratory of Lymphatic Surgery Jilin Province Changchun 130031 P. R. China.
College of Chemistry, Jilin University Changchun 130021 China.
RSC Adv. 2019 Dec 16;9(71):41431-41437. doi: 10.1039/c9ra08555f. eCollection 2019 Dec 13.
Near-infrared (NIR) fluorescent probes are widely employed in biological detection because of their lower damage to biological samples, low background interference, and high signal-to-noise ratio. Herein, a highly water-soluble NIR probe (NIRHA) based on a hemicyanine skeleton and bearing an acrylate moiety was synthesized. The probe showed high selectivity toward cysteine (Cys) over homocysteine (Hcy) and glutathione (GSH). The probe also had low cytotoxicity and was successfully applied in HeLa cells and mouse experiments. Results of bioimaging experiments indicated that the probe was effective for visualizing endogenous Cys and .
近红外（NIR）荧光探针因其对生物样品损伤小、背景干扰低、信噪比高而被广泛应用于生物检测。在此，合成了一种基于半菁骨架并带有丙烯酸酯部分的高水溶性近红外探针（NIRHA）。该探针对半胱氨酸（Cys）的选择性高于同型半胱氨酸（Hcy）和谷胱甘肽（GSH）。该探针还具有低细胞毒性，并成功应用于HeLa细胞和小鼠实验。生物成像实验结果表明，该探针可有效可视化内源性Cys 以及 。
