College of Laboratory Medicine, Hebei North University, 11 Diamond Street South, Zhangjiakou 075000, Hebei Province, China.
College of Economics and Management, Hebei North University, 11 Diamond Street South, Zhangjiakou 075000, Hebei Province, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Sep 5;202:301-304. doi: 10.1016/j.saa.2018.05.060. Epub 2018 May 16.
In this work, we have designed and synthesized the fluorescent probe 1, which showed a highly selective and sensitive response to Cys over Hcy/GSH in the test. Moreover, the color of probe solution has changed dramatically from colorless to pink with the addition of Cys within 10 min. Meanwhile, the fluorescence intensity exhibited perfectly positive correlation with concentration of Cys from 0 to 200 μM, which offered the important condition for quantitative analysis. Finally, the bioimaging and fluorescence response of probe 1 for fetal calf serum are a powerful safeguard for practical detection of Cys. Therefore, this near-infrared probe will be of great benefit for detecting Cys in the biological systems.
在这项工作中，我们设计并合成了荧光探针 1，它在测试中对 Cys 表现出高度选择性和敏感性的响应。此外，探针溶液的颜色在 10 分钟内从无色变为粉红色，随着 Cys 的加入而发生剧烈变化。同时，荧光强度与 Cys 的浓度从 0 到 200 μM 呈完全正相关，为定量分析提供了重要条件。最后，探针 1 对胎牛血清的生物成像和荧光响应为实际检测 Cys 提供了有力的保障。因此，这种近红外探针将非常有利于检测生物体系中的 Cys。
