Tian Yong, He Jingchun, Liu Nan, Huang Di, Liu Zhuo, Yang Yanrong, Chen Junyu, Zhao Benzheng, Zhao Shuhua, Liang Bing
School of Nursing, Jilin University Changchun 130021 Jilin China.
Basic Medical College, Jilin University Changchun 130021 Jilin China.
RSC Adv. 2018 Jun 13;8(39):21759-21767. doi: 10.1039/c8ra02671h.
Atrazine (ATZ), a widely used triazine herbicide, has been detected in the surface and ground water even far from where it is applied. Recently, the biotoxicity of atrazine to the immune, reproductive and endocrine systems has been preliminarily observed in laboratory experiments and epidemiological research studies. In order to further comprehend the carcinogenic nature of ATZ, and models were established in this study to explore the effects of ATZ exposure on hepatocellular carcinoma. The results showed that after being treated with ATZ, the proliferation of H22 cells increased, and the tumor volume and amount of ascites were significantly increased in an transplantation tumor model established in C57BL/6 mice compared to the control group. The expression of p53 was down-regulated, while the expression of cyclin-D1, VEGF, MMP2, Stat3 and C-myc was up-regulated in the ATZ-treated groups compared to the control group. These results indicate that ATZ might activate the Stat3 signaling pathway and promote the proliferation and invasion of hepatocellular carcinoma cells.
阿特拉津（ATZ）是一种广泛使用的三嗪类除草剂，即便在远离其施用地点的地表水和地下水中也能被检测到。最近，在实验室实验和流行病学研究中已初步观察到阿特拉津对免疫、生殖和内分泌系统的生物毒性。为了进一步了解ATZ的致癌特性，本研究建立了模型以探讨ATZ暴露对肝细胞癌的影响。结果显示，用ATZ处理后，H22细胞的增殖增加，与对照组相比，在C57BL/6小鼠建立的移植瘤模型中肿瘤体积和腹水量显著增加。与对照组相比，在经ATZ处理的组中p53的表达下调，而细胞周期蛋白D1、血管内皮生长因子（VEGF）、基质金属蛋白酶2（MMP2）、信号转导和转录激活因子3（Stat3）和原癌基因C-myc的表达上调。这些结果表明，ATZ可能激活Stat3信号通路并促进肝癌细胞的增殖和侵袭。
