Yin Yongqi, Qi Fei, Gao Lu, Rao Shengqi, Yang Zhenquan, Fang Weiming
College of Food Science and Technology, Yangzhou University Yangzhou Jiangsu 210095 People's Republic of China
RSC Adv. 2018 May 16;8(32):17905-17913. doi: 10.1039/c8ra02996b. eCollection 2018 May 14.
Soybean germination under stressful conditions, especially salt stress, has been verified to be an effective way of accumulating gamma-aminobutyric acid (GABA) in dark-germinated soybeans. In this study, a combination of physiological characteristics and isobaric tags for relative and absolute quantitation (iTRAQ) in a proteomic-based approach was used to investigate the protein changes in dark-germinated soybeans under salt stress. A total of 201 differentially abundant proteins (DAPs) were identified and divided into 13 functional groups. Under salt stress, 20 metabolic pathways were significantly enriched in dark-germinated soybeans. GABA content and antioxidase activity were increased while the growth and development of soybeans were inhibited by the salt stress. Promoting the synthesis of ROS-scavenging enzymes, maintaining the protein metabolic balance and re-establishing cellular homeostasis were very important strategies for growth stimulation in response to salt stress. In summary, these results showed comprehensive proteome coverage of dark-germinated soybeans in response to salt treatment, and increased our understanding of the molecular processes involved in plant networks responding to stresses.
在胁迫条件下，尤其是盐胁迫下，大豆发芽已被证实是在黑暗发芽大豆中积累γ-氨基丁酸（GABA）的有效方法。在本研究中，采用基于蛋白质组学的方法，结合生理特性和相对与绝对定量的等压标签（iTRAQ），研究盐胁迫下黑暗发芽大豆的蛋白质变化。共鉴定出201种差异丰度蛋白（DAP），并分为13个功能组。在盐胁迫下，黑暗发芽大豆中有20条代谢途径显著富集。盐胁迫抑制了大豆的生长发育，但GABA含量和抗氧化酶活性增加。促进活性氧清除酶的合成、维持蛋白质代谢平衡和重新建立细胞稳态是响应盐胁迫促进生长的重要策略。总之，这些结果显示了黑暗发芽大豆对盐处理的全面蛋白质组覆盖，并增进了我们对植物网络响应胁迫所涉及分子过程的理解。
