Shin Cheong, Lee Ha Neul, Ryu Jea Sung, Chung Hyun Jung
Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology 291 University Rd. Daejeon 34141 Korea
Department of Biological Sciences, Korea Advanced Institute of Science and Technology 291 University Rd. Daejeon 34141 Korea.
RSC Adv. 2018 Jul 12;8(44):25094-25103. doi: 10.1039/c8ra03540g. eCollection 2018 Jul 9.
Development of a rapid, point-of-care assay for diagnosing bacterial infections is crucial for subsequent treatment of the patient and preventing the overuse of antibiotics. Herein, we describe a rapid, one-step colorimetric assay based on the formation of nano-aggregates using nanobeads targeting Gram-positive bacteria. Vancomycin was immobilized onto blue-colored polymeric nanobeads to induce specific and multivalent binding with the Gram-positive bacterial cell wall and subsequent agglomeration. Without any pre-processing steps, the addition of various types of Gram-positive pathogens to the nanobeads resulted in the formation of blue precipitates, which could be observed with the naked eye in ∼30 min. We also utilized a porous filter system for the assay, which allowed discrimination of Gram-positive targets with higher selectivity, and demonstrated feasibility as a simple diagnostic assay with minimal technical components. We anticipate that the nanobead aggregation assay can be potentially applied as a rapid and simple sensing platform, which can be easily miniaturized and enable point-of-care diagnosis of Gram-positive infections.
开发一种用于诊断细菌感染的快速即时检测方法对于患者的后续治疗以及防止抗生素的过度使用至关重要。在此，我们描述了一种基于使用靶向革兰氏阳性菌的纳米珠形成纳米聚集体的快速一步比色检测方法。万古霉素固定在蓝色聚合物纳米珠上，以诱导与革兰氏阳性菌细胞壁的特异性多价结合并随后聚集。无需任何预处理步骤，将各种类型的革兰氏阳性病原体添加到纳米珠中会导致形成蓝色沉淀，在约30分钟内可用肉眼观察到。我们还将多孔过滤系统用于该检测，其允许以更高的选择性区分革兰氏阳性靶标，并证明了作为一种具有最少技术组件的简单诊断检测方法的可行性。我们预计纳米珠聚集检测方法有可能作为一种快速且简单的传感平台应用，该平台可以轻松小型化并实现革兰氏阳性感染的即时诊断。
