Department of Chemical Engineering, University of Waterloo, 200 University Avenue W, Waterloo, Ontario N2L 3G1, Canada; Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue W, Waterloo, Ontario N2L 3G1, Canada.
Department of Chemical Engineering, University of Waterloo, 200 University Avenue W, Waterloo, Ontario N2L 3G1, Canada; Center for Contact Lens Research, University of Waterloo, 200 University Avenue W, Waterloo, Ontario N2L 3G1, Canada.
Biotechnol Adv. 2015 Nov 1;33(6 Pt 1):666-80. doi: 10.1016/j.biotechadv.2015.03.003. Epub 2015 Mar 17.
Rapid detection of pathogens is crucial to minimize adverse health impacts of nosocomial, foodborne, and waterborne diseases. Gold nanoparticles are extremely successful at detecting pathogens due to their ability to provide a simple and rapid color change when their environment is altered. Here, we review general strategies of implementing gold nanoparticles in colorimetric biosensors. First, we highlight how gold nanoparticles have improved conventional genomic analysis methods by lowering detection limits while reducing assay times. Then, we focus on emerging point-of-care technologies that aim at pathogen detection using simpler assays. These advances will facilitate the implementation of gold nanoparticle-based biosensors in diverse environments throughout the world and help prevent the spread of infectious diseases.
快速检测病原体对于减轻医院感染、食源性和水源性疾病对健康的不利影响至关重要。由于金纳米粒子能够在环境发生变化时提供简单而快速的颜色变化，因此在检测病原体方面非常成功。在这里，我们综述了将金纳米粒子应用于比色生物传感器的一般策略。首先，我们强调了金纳米粒子如何通过降低检测限和缩短分析时间来改善传统的基因组分析方法。然后，我们专注于新兴的即时检测技术，这些技术旨在通过更简单的检测方法来检测病原体。这些进展将促进基于金纳米粒子的生物传感器在世界各地不同环境中的应用，并有助于防止传染病的传播。
