CAS Center for Excellence in Nanoscience, CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, Beijing Engineering Research Center for BioNanotechnology, National Center for NanoScience and Technology, ZhongGuanCun BeiYiTiao, Beijing, 100190, China.
Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, 610041, China.
Theranostics. 2018 Feb 4;8(5):1449-1457. doi: 10.7150/thno.22540. eCollection 2018.
Rapid and facile detection of pathogenic bacteria is challenging due to the requirement of large-scale instruments and equipment in conventional methods. We utilize D-amino acid as molecules to selectively target bacteria because bacteria can incorporate DADA in its cell wall while mammalian cells or fungi cannot. We show a broad-spectrum bacterial detection system based on D-amino acid-capped gold nanoparticles (AuNPs). AuNPs serve as the signal output that we can monitor without relying on any complex instruments. In the presence of bacteria, the AuNPs aggregate and the color of AuNPs changes from red to blue. This convenient color change can distinguish between () and methicillin-resistant (MRSA). This system can be applied for detection of ascites samples from patients. These D-amino acid-modified AuNPs serve as a promising platform for rapid visual identification of pathogens in the clinic.
由于传统方法需要大规模的仪器和设备，因此快速、简便地检测致病菌具有挑战性。我们利用 D-氨基酸作为分子来选择性地靶向细菌，因为细菌可以将 DADA 掺入其细胞壁中，而哺乳动物细胞或真菌则不能。我们展示了一种基于 D-氨基酸封端的金纳米粒子 (AuNP) 的广谱细菌检测系统。AuNP 作为信号输出，我们可以在不依赖任何复杂仪器的情况下进行监测。在细菌存在的情况下，AuNP 聚集，AuNP 的颜色从红色变为蓝色。这种方便的颜色变化可以区分金黄色葡萄球菌（ ）和耐甲氧西林金黄色葡萄球菌（MRSA）。该系统可用于检测来自患者的腹水样本。这些 D-氨基酸修饰的 AuNP 为临床中快速目视识别病原体提供了一个很有前途的平台。
