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一种基于双电子转移/分子内电荷转移机制的用于选择性检测半胱氨酸的简单双光子开启荧光探针。

A simple two-photon turn-on fluorescent probe for the selective detection of cysteine based on a dual PeT/ICT mechanism.

作者信息

Chen Xiani, Xu Hang, Ma Shengnan, Tong Hongjuan, Lou Kaiyan, Wang Wei

机构信息

Shanghai Key Laboratory of New Drug Design, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, State Key Laboratory of Bioengineering Reactor, East China University of Science and Technology 130 Meilong Road Shanghai 200237 China

School of Pharmacy, Shaanxi Institute of International Trade & Commerce Xi'an 712046 China.

出版信息

RSC Adv. 2018 Apr 10;8(24):13388-13392. doi: 10.1039/c8ra02138d. eCollection 2018 Apr 9.

DOI:10.1039/c8ra02138d
PMID:35542560
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9079754/
Abstract

Herein, a simple two-photon turn-on fluorescent probe, -(6-acyl-2-naphthayl)-maleimide (1), based on a dual PeT/ICT quenching mechanism is reported for the highly sensitive and selective detection of cysteine (Cys) over other biothiols. The probe was applied in the two-photon imaging of Cys in cultured HeLa cells, excited by a near-infrared laser at 690 nm.

摘要

在此，报道了一种基于双电子转移/分子内电荷转移猝灭机制的简单双光子开启荧光探针-(6-酰基-2-萘基)-马来酰亚胺(1)，用于对胱氨酸（Cys）进行高灵敏度和高选择性检测，以区别于其他生物硫醇。该探针应用于培养的HeLa细胞中Cys的双光子成像，由690 nm的近红外激光激发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1938/9079754/fa4bf3fa7da4/c8ra02138d-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1938/9079754/2059f016c62c/c8ra02138d-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1938/9079754/eaf9d4a89164/c8ra02138d-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1938/9079754/fa4bf3fa7da4/c8ra02138d-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1938/9079754/2059f016c62c/c8ra02138d-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1938/9079754/eaf9d4a89164/c8ra02138d-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1938/9079754/fa4bf3fa7da4/c8ra02138d-f3.jpg

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2
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J Org Chem. 2017 Dec 1;82(23):12153-12161. doi: 10.1021/acs.joc.7b01952. Epub 2017 Nov 20.
3
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4
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Chemistry. 2020 Dec 4;26(68):15867-15870. doi: 10.1002/chem.202003951. Epub 2020 Oct 27.
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4
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