Brozek-Pluska Beata, Miazek Krystian, Musiał Jacek, Kordek Radzislaw
Laboratory of Laser Molecular Spectroscopy, Institute of Applied Radiation Chemistry, Lodz University of Technology Wroblewskiego 15 93-590 Lodz Poland
Department of Pathology, Chair of Oncology, Medical University of Lodz Paderewskiego 4 92-213 Lodz Poland.
RSC Adv. 2019 Dec 6;9(69):40445-40454. doi: 10.1039/c9ra06831g. eCollection 2019 Dec 3.
Raman spectroscopy and imaging are highly structure-sensitive methods that allow the characterization of biological samples with minimal impact. In this paper, Raman spectra and imaging of noncancerous and cancerous human colon tissue samples were measured at different excitation wavelengths: 355, 532, and 785 nm. Intra-patient variability in the analyzed spectra showed colon sample heterogeneity for both noncancerous and cancerous human sample types. The lowest inter-patient variability of Raman spectra was observed for the fingerprint region of noncancerous samples for the 532 nm excitation laser line. The bands of principal biochemical constituents (proteins, lipids, nucleic acids) predominate in VIS and NIR-Raman spectra (excitation: 532, 785 nm), with the special role of the bands of intrinsic tissue chromophores-carotenoids for VIS excitation due to resonance enhancement. At 355 nm excitation, high autofluorescence of colon tissues were observed. Our studies proved high potential of Raman spectroscopy and Raman imaging in differentiation of noncancerous and cancerous human colon tissues and that the wavelengths 532 and 785 nm offer wide possibilities for the detection of human colon tissue pathology for and measurements and prevail over 355 nm excitation.
拉曼光谱和成像技术是高度结构敏感的方法，能够在对生物样品产生最小影响的情况下对其进行表征。在本文中，在不同激发波长（355、532和785nm）下测量了非癌和癌性人类结肠组织样品的拉曼光谱和成像。分析光谱中的患者内变异性表明，非癌和癌性人类样品类型的结肠样品均具有异质性。对于532nm激发激光线，在非癌样品的指纹区域观察到拉曼光谱的患者间变异性最低。主要生化成分（蛋白质、脂质、核酸）的谱带在可见和近红外拉曼光谱（激发波长：532、785nm）中占主导地位，由于共振增强，固有组织发色团——类胡萝卜素的谱带在可见激发中具有特殊作用。在355nm激发下，观察到结肠组织的高自发荧光。我们的研究证明了拉曼光谱和拉曼成像在区分非癌和癌性人类结肠组织方面具有很高的潜力，并且532和785nm波长为检测人类结肠组织病理学以及测量提供了广泛的可能性，优于355nm激发。
