Frank C J, Redd D C, Gansler T S, McCreery R L
Department of Chemistry, Ohio State University, Columbus 43210.
Anal Chem. 1994 Feb 1;66(3):319-26. doi: 10.1021/ac00075a002.
Breast biopsy samples were examined with Raman spectroscopy with laser wavelengths ranging from 406 to 830 nm. A combination of a single-stage spectrograph, band reject filter, and CCD detector permitted low laser powers and minimal risk of sample radiation damage. Spectra of formalin-fixed human tissue revealed Raman features for lipids and carotenoids. The best defined lipid features were observed for 782- and 830-nm laser excitation, while carotenoid features were strongest in the 488-515-nm range due to resonance enhancement. Comparison of the spectra with those of fatty acid esters revealed that the major lipid component is a derivative of oleic acid. Lipid and carotenoid Raman bands were superimposed on a luminescent background which was less prominent at longer laser wavelengths. A compact, portable, diode laser spectrometer was tested in a clinical setting with fiber optic sampling. The results indicate that substantial biochemical information is available from near-IR Raman spectroscopy and the technique may have clinical applications.
采用激光波长范围为406至830nm的拉曼光谱对乳腺活检样本进行检查。单级光谱仪、带阻滤波器和电荷耦合器件（CCD）探测器的组合使得激光功率较低，且样本受辐射损伤的风险最小。福尔马林固定的人体组织光谱显示出脂质和类胡萝卜素的拉曼特征。在782和830nm激光激发下观察到最清晰的脂质特征，而由于共振增强，类胡萝卜素特征在488 - 515nm范围内最强。将这些光谱与脂肪酸酯的光谱进行比较后发现，主要脂质成分是油酸的衍生物。脂质和类胡萝卜素的拉曼谱带叠加在一个发光背景上，该背景在较长激光波长下不太明显。在临床环境中使用光纤采样对一台紧凑、便携的二极管激光光谱仪进行了测试。结果表明，近红外拉曼光谱可提供大量生化信息，该技术可能具有临床应用价值。
