Staniszewska-Slezak Emilia, Malek Kamilla, Baranska Malgorzata
Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland; Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland.
Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland; Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Aug 5;147:245-56. doi: 10.1016/j.saa.2015.03.086. Epub 2015 Mar 28.
Raman spectroscopy and four excitation lines in the visible (Vis: 488, 532, 633 nm) and near infrared (NIR: 785 nm) were used for biochemical analysis of rat tissue homogenates, i.e. myocardium, brain, liver, lung, intestine, and kidney. The Vis Raman spectra are very similar for some organs (brain/intestines and kidney/liver) and dominated by heme signals when tissues of lung and myocardium were investigated (especially with 532 nm excitation). On the other hand, the NIR Raman spectra are specific for each tissue and more informative than the corresponding ones collected with the Vis excitations. The spectra analyzed without any special pre-processing clearly illustrate different chemical composition of each tissue and give information about main components e.g. lipids or proteins, but also about the content of some specific compounds such as amino acid residues, nucleotides and nucleobases. However, in order to obtain the whole spectral information about tissues complex composition the spectra of Vis and NIR excitations should be collected and analyzed together. A good agreement of data gathered from Raman spectra of the homogenates and those obtained previously from Raman imaging of the tissue cross-sections indicates that the presented here approach can be a method of choice for an investigation of biochemical variation in animal tissues. Moreover, the Raman spectral profile of tissue homogenates is specific enough to be used for an investigation of potential pathological changes the organism undergoes, in particular when supported by the complementary FTIR spectroscopy.
拉曼光谱以及可见光（Vis：488、532、633 nm）和近红外光（NIR：785 nm）中的四条激发线被用于大鼠组织匀浆（即心肌、脑、肝、肺、肠和肾）的生化分析。对于某些器官（脑/肠以及肾/肝），可见拉曼光谱非常相似，而在研究肺和心肌组织时（特别是用532 nm激发），光谱以血红素信号为主。另一方面，近红外拉曼光谱对每种组织都具有特异性，并且比用可见光激发收集的相应光谱更具信息性。未经任何特殊预处理分析的光谱清楚地说明了每种组织的不同化学成分，并给出了有关主要成分（例如脂质或蛋白质）的信息，还给出了有关某些特定化合物（如氨基酸残基、核苷酸和核碱基）含量的信息。然而，为了获得有关组织复杂组成的完整光谱信息，应同时收集和分析可见光和近红外激发的光谱。从匀浆的拉曼光谱收集的数据与先前从组织横截面的拉曼成像获得的数据高度吻合，这表明本文提出的方法可以作为研究动物组织生化变化的一种选择方法。此外，组织匀浆的拉曼光谱特征足够特异，可用于研究生物体所经历的潜在病理变化，特别是在辅以互补的傅里叶变换红外光谱时。
