Wang Weiguo, Zhu Sailing, Zhang Guoqing, Wu Fan, Ban Jinghao, Wang Limin
Department of Stomatology, No. 903 Hospital of PLA Lingyin Road 14 Hangzhou 310000 People's Republic of China
School of Stomatology, Fourth Military Medical University Xi'an People's Republic of China.
RSC Adv. 2019 Dec 9;9(69):40681-40688. doi: 10.1039/c9ra07788j. eCollection 2019 Dec 3.
Resins with strong antibacterial and thermomechanical properties are critical for application in oral cavities. In this study, we first evaluated the antibacterial effect of an unfilled resin incorporating 1, 4, and 7 mass% of quaternary ammonium salt (QAS) monomers containing two methacrylate groups (MAE-DB) and four methacrylate groups (TMH-DB) against , and tested the cytotoxicity and thermomechanical properties of the 4 mass% MAE-DB and TMH-DB modified resin materials. A neat resin without a QAS monomer served as the control. As the concentration of both QAS monomers increases, the formation of a biofilm on the experimental material is increasingly inhibited. The results of colony forming unit counts and the metabolic activity showed that both the MAE-DB and TMH-DB modified resins have a strong bactericidal effect on the bacteria in a biofilm, but no bactericidal effect on the bacteria in a solution. The viability-staining and morphology results also demonstrate that the bacteria deform, lyse, shrink, and die on the surface of the two QAS-modified resins. Cytotoxicity results show that the addition of TMH-DB can reduce the cytotoxicity of the resin, while the addition of MAE-DB increases the cytotoxicity of the resin. DMA results show that a TMH-DB modified resin has a higher storage modulus than a MAE-DB modified resin owing to its better crosslink density. The two groups of experimental resins showed a similar glass transition temperature. These data indicate that the two QAS monomers can impart similar antibacterial properties upon contact with a dental resin, whereas TMH-DB can endow the resin with a higher crosslink density and storage modulus than MAE-DB because it has more polymerizable groups.
具有强大抗菌和热机械性能的树脂对于口腔应用至关重要。在本研究中，我们首先评估了一种未填充树脂（含有1%、4%和7%质量分数的含两个甲基丙烯酸酯基团（MAE-DB）和四个甲基丙烯酸酯基团（TMH-DB）的季铵盐（QAS）单体）对[具体细菌未给出]的抗菌效果，并测试了4%质量分数的MAE-DB和TMH-DB改性树脂材料的细胞毒性和热机械性能。不含QAS单体的纯树脂用作对照。随着两种QAS单体浓度的增加，实验材料上生物膜的形成受到越来越强的抑制。菌落形成单位计数和代谢活性结果表明，MAE-DB和TMH-DB改性树脂对生物膜中的细菌均有很强的杀菌作用，但对溶液中的细菌无杀菌作用。活细胞染色和形态学结果也表明，细菌在两种QAS改性树脂表面会变形、裂解、收缩并死亡。细胞毒性结果表明，添加TMH-DB可降低树脂的细胞毒性，而添加MAE-DB则会增加树脂的细胞毒性。动态热机械分析（DMA）结果表明，由于TMH-DB具有更好的交联密度，其改性树脂比MAE-DB改性树脂具有更高的储能模量。两组实验树脂显示出相似的玻璃化转变温度。这些数据表明，两种QAS单体与牙科树脂接触时可赋予相似的抗菌性能，而TMH-DB比MAE-DB能使树脂具有更高交联密度和储能模量，因为它具有更多可聚合基团。
