Zhang Xia, Liang Caijuan, Yin Jintuo, Sun Yupeng, Zhang Lantong
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University Shijiazhuang 050017 P. R. China
RSC Adv. 2018 Mar 27;8(21):11813-11827. doi: 10.1039/c7ra13760e. eCollection 2018 Mar 21.
Liquiritin (LQ), the main bioactive constituent of licorice, is a common flavoring and sweetening agent in food products and has a wide range of pharmacological properties, including antidepressant-like, neuroprotective, anti-cancer and anti-inflammatory properties. This study investigated the metabolic pathways of LQ (rat liver microsomes) and (rat model) using ultra high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS). Moreover, supplementary tools such as key product ions (KPIs) were employed to search for and identify compounds. As a result, 56 metabolites and 15 metabolites were structurally characterized. Oxidation, reduction, hydrolysis, methylation, acetylation, and sulfate and glucuronide conjugation were determined to be the major metabolic pathways of LQ, and there were differences in LQ metabolism and . In addition, the and metabolic pathways were compared in this study.
甘草苷（LQ）是甘草的主要生物活性成分，是食品中常见的调味剂和甜味剂，具有广泛的药理特性，包括抗抑郁样、神经保护、抗癌和抗炎特性。本研究使用超高效液相色谱与混合三重四极杆飞行时间质谱联用技术（UHPLC-Q-TOF-MS/MS）研究了LQ在大鼠肝微粒体和大鼠模型中的代谢途径。此外，还使用了关键产物离子（KPI）等辅助工具来搜索和鉴定化合物。结果，对56种代谢物和15种代谢物进行了结构表征。氧化、还原、水解、甲基化、乙酰化以及硫酸化和葡萄糖醛酸化结合被确定为LQ的主要代谢途径，并且LQ在大鼠肝微粒体和大鼠模型中的代谢存在差异。此外，本研究还比较了大鼠肝微粒体和大鼠模型的代谢途径。
